Institute of Microbiology, The Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic.
Institute of Microbiology, The Academy of Sciences of the Czech Republic, Centre Algatech, Laboratory of Cell Cycles of Algae, Opatovický mlýn 237, 379 81 Třeboň, Czech Republic.
J Chromatogr A. 2019 Nov 8;1605:460365. doi: 10.1016/j.chroma.2019.460365. Epub 2019 Jul 23.
The analysis of triacylglycerols and phospholipids - phosphatidylcholines allowed the use of shotgun lipidomics to identify very long-chain fatty acids and very long-chain polyunsaturated fatty acids in microalgae. These fatty acids were determined in triacylglycerols by positive electrospray ionization of neutral loss scans of different fatty acids, e.g. 24:0, 24:1ω9, 24:6ω3, 26:0, 26:1ω9, 28:0, 28:1ω9, 28:2ω6, and 28:8ω3. Likewise, very long-chain fatty acids in phosphatidylcholines were identified by negative electrospray ionization mass spectrometry in the selected ion-monitoring of the two most important ions (RCOO and RCOO). The limit of detection was determined at 10 nmol/L (∼11 pg/μL) in triacylglycerols and 8.6 nmoles/L (∼8 pg/μL) in phosphatidylcholines. The use of liquid chromatography-mass spectrometry is suitable for very long-chain polyunsaturated fatty acids with up to 8 double bonds due to the time of analysis as well as for reasons of lower thermal stability of polyunsaturated fatty acids towards saturated fatty acids, but gas chromatography-mass spectrometry is better suited for the analysis of saturated very long-chain fatty acids.
三酰基甘油和磷脂 - 磷脂酰胆碱的分析使我们能够使用 shotgun 脂质组学在微藻中鉴定出非常长链脂肪酸和非常长链多不饱和脂肪酸。这些脂肪酸是通过对不同脂肪酸的中性丢失扫描进行正电喷雾电离，例如 24:0、24:1ω9、24:6ω3、26:0、26:1ω9、28:0、28:1ω9、28:2ω6 和 28:8ω3，在三酰基甘油中确定的。同样，通过负电喷雾电离质谱在两个最重要的离子（RCOO 和 RCOO）的选择离子监测中鉴定出磷脂酰胆碱中的非常长链脂肪酸。在三酰甘油中的检测限为 10 nmol/L（约 11 pg/μL），在磷脂酰胆碱中的检测限为 8.6 nmol/L（约 8 pg/μL）。由于分析时间以及多不饱和脂肪酸相对于饱和脂肪酸的较低热稳定性等原因，液相色谱-质谱法适用于多达 8 个双键的非常长链多不饱和脂肪酸，但气相色谱-质谱法更适合分析饱和的非常长链脂肪酸。
