体外培养因素对细胞培养中甜菊糖苷生长和生物合成的影响。
Impact of cultivation factors in vitro on the growth and the biosynthesis of steviol glycosides in cell cultures.
作者信息
Bondarev Nikolai, Reshetnyak Oxana, Bondareva Tatyana, Il'in Michael, Nosov Alexander
机构信息
1Department of Industrial Chemistry and Biotechnology, Orel State University named after IS Turgenev, Orel, Russia 302026.
2KA Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia 127276.
出版信息
Physiol Mol Biol Plants. 2019 Jul;25(4):1091-1096. doi: 10.1007/s12298-019-00680-6. Epub 2019 Jun 19.
The growth and the biosynthesis of steviol glycosides (SGs) in cell cultures subjected to the impact of different cultivation factors were investigated under in vitro conditions. The changes in composition and concentration of constituted components of the nutrient medium was found to exert a significant influence on the of cell culture growth and the biosynthesis of SGs. It was found that combination of growth regulators in the nutrient medium is more important factor for proliferation of isolated cells of while the level of SGs accumulation depends to greater extent on genotypical features of cell strain. Light was shown to activate the biosynthesis of SGs, providing evidence for the suggestion about chloroplasts participation in this activation. In morphogenic callus cultures, the content of SGs was about ten times higher, indicating that the level of the accumulation of the compounds in question depends on cell differentiation and specialization.
在体外条件下，研究了不同培养因素对细胞培养物中甜菊糖苷（SGs）生长和生物合成的影响。发现营养培养基组成成分的变化对细胞培养物的生长和SGs的生物合成有显著影响。结果表明，营养培养基中生长调节剂的组合是分离细胞增殖的更重要因素，而SGs的积累水平在更大程度上取决于细胞株的基因型特征。光照被证明可激活SGs的生物合成，为叶绿体参与这种激活作用的推测提供了证据。在形态发生愈伤组织培养物中，SGs的含量高出约10倍，表明上述化合物的积累水平取决于细胞分化和特化。
Zotero 插件