Bondarev Nikolai, Reshetnyak Oxana, Nosov Alexander
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia.
Planta Med. 2002 Aug;68(8):759-62. doi: 10.1055/s-2002-33809.
Growth and development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides (SGs) were investigated. It was found that, owing to the highly favorable conditions of shoot cultivation created in such an apparatus, the intensity of shoot growth and SG production appeared to be 1.5 - 2.0 times higher than those of the shoots grown in tubes. These results indicate the existence of a positive correlation between these two processes. The data obtained suggest that the enhanced SG production is due to the differentiation of chlorenchyma cells and formation of specific subcellular structures for the glycoside to be accumulated.
研究了在滚筒生物反应器中培养的甜叶菊嫩枝的生长发育及其甜菊糖苷（SGs）的产生。结果发现，由于在这种装置中创造了非常有利于嫩枝培养的条件，嫩枝的生长强度和SG的产生似乎比在试管中生长的嫩枝高1.5 - 2.0倍。这些结果表明这两个过程之间存在正相关。所获得的数据表明，SG产量的提高是由于薄壁组织细胞的分化以及积累糖苷的特定亚细胞结构的形成。
