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采用酶联免疫吸附测定法检测尼日利亚血清中的黄曲霉毒素。

Measurement of aflatoxin in Nigerian sera by enzyme-linked immunosorbent assay.

作者信息

Denning D W, Onwubalili J K, Wilkinson A P, Morgan M R

机构信息

Lister Unit, Northwick Park Hospital, Harrow Middlesex, UK.

出版信息

Trans R Soc Trop Med Hyg. 1988;82(1):169-71. doi: 10.1016/0035-9203(88)90299-4.

Abstract

Sera from 78 healthy men donating blood in Enugu, Nigeria were examined for aflatoxin by enzyme-linked immunosorbent assay (ELISA). Levels varied from less than 20 pg/ml to 3.1 ng/ml. The ELISA method is simple, sensitive and specific, and therefore well suited to the low-resource tropical environment. Aflatoxin is ingested in considerable amounts in Nigeria and many contribute, with hepatitis B, to the development of hepatocellular carcinoma.

摘要

采用酶联免疫吸附测定法(ELISA)对来自尼日利亚埃努古的78名献血健康男性的血清进行黄曲霉毒素检测。检测水平从低于20 pg/ml到3.1 ng/ml不等。ELISA方法简单、灵敏且特异,因此非常适合资源匮乏的热带环境。在尼日利亚,人们大量摄入黄曲霉毒素,许多人还同时感染乙肝,这两者共同导致肝细胞癌的发生。

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