Department of Neurosurgery, Qilu Hospital of Shandong University (Qing Dao), Shandong, China.
Department of Endocrinology, Qilu Hospital of Shandong University (Qing Dao), Shandong, China.
World Neurosurg. 2019 Dec;132:e795-e801. doi: 10.1016/j.wneu.2019.08.008. Epub 2019 Aug 9.
To examine changes of expression and activity of phosphodiesterase V (PDE V) in the basilar artery following cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) in a rabbit model.
A rabbit model of CVS after SAH was constructed by double blood injection into the cisterna magna. Subjects were divided into 3 groups: blank control group, normal saline group, and SAH group. Transcranial Doppler and selective vertebrobasilar digital subtraction angiography were performed to identify changes of CVS. Changes of PDE V expression and activity were examined.
Mean basilar arterial blood flow rate measured by transcranial Doppler was significantly increased in the SAH group compared with the blank control group and normal saline group. Mean basilar artery diameter measured by digital subtraction angiography in the SAH group was narrower than in the other 2 groups. Compared with the other 2 groups, the expression of PDE V in the SAH group was significantly upregulated, and the activity was significantly enhanced.
The rabbit model of SAH-induced CVS was successfully constructed through double blood injection method. Increased basilar artery blood flow, narrowing of the basilar artery, increased PDE V expression, and enhanced PDE V activity in the basilar artery were detected in the CVS rabbits, suggesting that PDE V has the potential to be used as a target for CVS therapy.
研究兔蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）模型基底动脉中磷酸二酯酶 V（PDE V）表达和活性的变化。
采用枕大池二次注血法建立兔 CVS 模型。将实验动物分为 3 组：空白对照组、生理盐水组和 SAH 组。通过经颅多普勒超声和选择性椎基底动脉数字减影血管造影评估 CVS 的变化。检测 PDE V 表达和活性的变化。
与空白对照组和生理盐水组相比，SAH 组基底动脉血流速度明显增加。数字减影血管造影显示 SAH 组基底动脉直径较其他 2 组变窄。与其他 2 组相比，SAH 组 PDE V 的表达明显上调，活性明显增强。
通过二次注血法成功建立了兔 SAH 诱导的 CVS 模型。在 CVS 兔中检测到基底动脉血流增加、基底动脉变窄、PDE V 表达增加和 PDE V 活性增强，提示 PDE V 有可能成为 CVS 治疗的靶点。
