Kim Seongsoo, Lee Sang-Myung, Lee Sung Sik, Shin Dong-Sik
Division of Chemical and Bioengineering, Kangwon National University, Gangwon-do 24341, Korea.
Scientific Center for Optical and Electron Microscopy, ETH Zurich, CH-8093 Zurich, Switzerland.
Micromachines (Basel). 2019 Aug 9;10(8):527. doi: 10.3390/mi10080527.
Microfluidic generation of hydrogel microbeads is a highly efficient and reproducible approach to create various functional hydrogel beads. Here, we report a method to prepare crosslinked amino-functionalized polyethylene glycol (PEG) microbeads using a microfluidic channel. The microbeads generated from a microfluidic device were evaluated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and confocal laser scanning microscopy, respectively. We found that the microbeads were monodisperse and the amino groups were localized on the shell region of the microbeads. A swelling test exhibited compatibility with various solvents. A cell binding assay was successfully performed with RGD peptide-coupled amino-functionalized hydrogel microbeads. This strategy will enable the large production of the various functional microbeads, which can be used for solid phase peptide synthesis and on-bead bioassays.
微流控技术制备水凝胶微珠是一种高效且可重复的方法，用于创建各种功能性水凝胶珠。在此，我们报告一种使用微流控通道制备交联氨基功能化聚乙二醇（PEG）微珠的方法。分别通过扫描电子显微镜（SEM）、能量色散X射线光谱（EDS）和共聚焦激光扫描显微镜对微流控装置产生的微珠进行了评估。我们发现微珠是单分散的，并且氨基位于微珠的壳区域。溶胀测试表明其与各种溶剂具有相容性。使用RGD肽偶联的氨基功能化水凝胶微珠成功进行了细胞结合测定。该策略将能够大量生产各种功能性微珠，可用于固相肽合成和珠上生物测定。
Zotero 插件
