Shagam N L, Efremova V N, Kurbatova E A, Kaverina K G
Zh Mikrobiol Epidemiol Immunobiol. 1988 Jun(6):51-5.
S. aureus aqueous extract and K. pneumoniae hydroxylamine vaccine were studied by means of chemical and immunochemical analytical techniques. The preparations were found to contain, respectively, 7.0% nad 53.5% of neutral monosaccharides, 6.5% and 0.7% of nucleic acids, as well as protein in approximately equal amounts (11.63-14.0%). In experiment of immunodiffusion, immunoelectrophoresis and rocket immunoelectrophoresis in homologous systems with hyperimmune antimicrobial sera the preparations were characterized by serological heterogeneity. After their combination with Escherichia coli aqueous extract and Proteus hydroxylamine preparation their serological characteristics remaIned unchanged. The study of cross reactions of all components of the combined preparations with hyperimmune rabbit sera to the corresponding microorganisms revealed that only Klebsiella component of the combined vaccine reacted with all hyperimmune sera. The preparation of Proteus showed the lowest activity, it reacted only with hyperimmune sera to K. pneumoniae. Besides, no reaction of S. aureus component with sera to E. coli and no reaction of the preparation of E. coli with antistaphylococcal serum were observed.
采用化学和免疫化学分析技术对金黄色葡萄球菌水提取物和肺炎克雷伯菌羟胺疫苗进行了研究。结果发现，这些制剂分别含有7.0%和53.5%的中性单糖、6.5%和0.7%的核酸，以及含量大致相等的蛋白质（11.63 - 14.0%）。在与超免疫抗微生物血清的同源系统中进行免疫扩散、免疫电泳和火箭免疫电泳实验时，这些制剂表现出血清学异质性。它们与大肠杆菌水提取物和变形杆菌羟胺制剂结合后，血清学特征保持不变。对联合制剂的所有成分与针对相应微生物的超免疫兔血清的交叉反应研究表明，联合疫苗中只有克雷伯菌成分能与所有超免疫血清发生反应。变形杆菌制剂的活性最低，它仅与针对肺炎克雷伯菌的超免疫血清发生反应。此外，未观察到金黄色葡萄球菌成分与针对大肠杆菌的血清发生反应，也未观察到大肠杆菌制剂与抗葡萄球菌血清发生反应。
