Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, South Korea; Department of Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha 769008, India.
Department of Animal Science and Biotechnology, Sangji Youngseo College, Wonju 26339, South Korea.
Anim Reprod Sci. 2019 Sep;208:106118. doi: 10.1016/j.anireprosci.2019.106118. Epub 2019 Jul 9.
Treatment of donor cells and/or cloned embryos with cytidine analogues, having an Aza group at its 5th carbon (5-Aza), such as 5-Azacytidine (5-Aza-C) or 5-Aza-2'-deoxycytidine (5-Aza-dC) improves the in vitro development of cloned embryos produced by somatic cell nuclear transfer (SCNT). In vitro maturation (IVM) of immature pig oocytes treated with 5-Aza-C not only results in greater (P < 0.05) meiotic maturation to the MII stage but also enhances the capacity of 5-Aza-C treated oocytes for early embryonic development after parthenogenetic activation (PA), in vitro fertilization (IVF) or SCNT in a dose-dependent manner (0-10 μM). Cloned embryos generated from 5-Aza-C (0.01 μM) treated oocytes had an increased capacity to develop to the blastocyst stage (14.1 ± 1.5% compared with 9.6 ± 1.8%), greater probability of hatching (61.8 ± 1.5% compared with 45.0 ± 3.9%) and contained a greater number of cells per blastocyst (38.5 ± 4.4 compared with 30.5 ± 3.4) than those produced from non-treated control oocytes (P < 0.05). Data from the present study indicate that treatment of oocytes with 5-Aza-C may be an important approach to enhance the meiotic maturation and subsequent in vitro development of pig embryos. Future studies should be conducted to determine the underlying mechanism of improved early embryonic development of 5-Aza-C treated oocytes.
用 5-氮杂胞苷（5-Aza-C）或 5-氮杂-2'-脱氧胞苷（5-Aza-dC）等在其 5 号碳上具有氮杂基团的胞嘧啶类似物处理供体细胞和/或克隆胚胎可提高体细胞核移植（SCNT）产生的克隆胚胎的体外发育。用 5-Aza-C 处理不成熟猪卵母细胞的体外成熟（IVM）不仅导致更高的（P<0.05）减数分裂成熟到 MII 阶段，而且还增强了 5-Aza-C 处理卵母细胞在孤雌激活（PA）、体外受精（IVF）或 SCNT 后进行早期胚胎发育的能力，呈剂量依赖性（0-10μM）。从 5-Aza-C（0.01μM）处理的卵母细胞中产生的克隆胚胎具有更高的发育至囊胚阶段的能力（14.1±1.5%比 9.6±1.8%），更高的孵化概率（61.8±1.5%比 45.0±3.9%）和每个囊胚的细胞数更多（38.5±4.4 比 30.5±3.4），比未处理的对照组卵母细胞（P<0.05）。本研究的数据表明，用 5-Aza-C 处理卵母细胞可能是提高猪胚胎减数分裂成熟和随后体外发育的重要方法。未来的研究应确定 5-Aza-C 处理卵母细胞早期胚胎发育改善的潜在机制。
