Ponsano Elisa Helena Giglio, Grassi Thiago Luís Magnani, Santo Edson Francisco Espiríto, de Lima Leandro Kanamaru Franco, Pereira Raquel de Cássia
1Faculty of Veterinary Medicine, UNESP Univ Estadual Paulista, Araçatuba, SP Brazil.
Federal Institute of Education, Science and Technology, Manaus, AM Brazil.
3 Biotech. 2019 Sep;9(9):325. doi: 10.1007/s13205-019-1860-z. Epub 2019 Aug 8.
In this study, the cultivation of in fish industry effluent was carried out to cause the depollution of the by-product and generate a protein-carotenoid-rich biomass suitable to be used in tilapia feed. The bacterium was grown in the effluent (inoculum 1% v/v, 30 ± 5 °C, 2000 ± 500 lx, 7 days) and recovered as a biomass (microfiltration + centrifugation + lyophilization), resulting in a decrease of ca. 80% in the chemical oxygen demand of the industry by-product and so putting it within the required limits for wastewater discard in Brazil. The biomass was characterized as a nontoxic product (behavior and physiology data) provided of nutritional and technological properties due to its composition-46% protein, 17% lipid, 5% minerals and 0.3% red oxycarotenoids. When cultured tilapias were fed the biomass (0, 175, 350, 700 or 1400 mg/kg, 80 days), their fillets got redder and had increased protein and carotenoid contents. Feeding the tilapias with the biomass also delayed fillet rancidity up to 80 days of storage under freezing. Therefore, the biotechnological application of was demonstrated, providing a useful product and a service for the environment.
在本研究中,开展了利用渔业废水培养[细菌名称未给出]以实现副产物去污染并生成适合用于罗非鱼饲料的富含蛋白质 - 类胡萝卜素生物质的实验。该细菌在废水中培养(接种量1% v/v,30 ± 5 °C,2000 ± 500 lx,7天),并通过微滤 + 离心 + 冻干回收为生物质,使该工业副产物的化学需氧量降低了约80%,从而使其符合巴西废水排放的要求限值。该生物质被表征为无毒产品(行为和生理数据),因其组成(46%蛋白质、17%脂质、5%矿物质和0.3%红色氧化类胡萝卜素)而具有营养和技术特性。当给养殖的罗非鱼投喂该生物质(0、175、350、700或1400 mg/kg,80天)时,它们的鱼片颜色变红,蛋白质和类胡萝卜素含量增加。用该生物质喂养罗非鱼还将鱼片在冷冻储存下的酸败延迟至80天。因此,[细菌名称未给出]的生物技术应用得到了证明,为环境提供了有用的产品和服务。