Schwartz Egbert, Hayer Michaela, Hungate Bruce A, Mau Rebecca L
Department of Biological Sciences, Center for Ecosystem Science and Society, Northern Arizona University, Flagstaff, AZ, USA.
Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
Methods Mol Biol. 2019;2046:129-136. doi: 10.1007/978-1-4939-9721-3_10.
We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with HO. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled with O. By measuring how much the DNA of each taxon becomes enriched with O when an environmental sample is incubated with HO, it is feasible to quantify that population's DNA replication rate, which is a proxy for growth.
我们描述了一种通过用重水（H₂¹⁸O）进行稳定同位素探测（SIP）来研究环境样品中微生物生长的方案。水是所有微生物的通用底物，而DNA要被¹⁸O标记则需要进行复制。通过测量当环境样品与H₂¹⁸O一起孵育时每个分类群的DNA被¹⁸O富集的程度，就可以量化该种群的DNA复制速率，而这是生长的一个替代指标。
