Weis Severin, Schnell Sylvia, Egert Markus
Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene Group, Furtwangen University, Villingen-Schwenningen, Germany.
Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Giessen, Germany.
Methods Mol Biol. 2019;2046:221-231. doi: 10.1007/978-1-4939-9721-3_17.
The RNA-SIP technology allows for linking the structure and function of complex microbial communities, that is, the identification of microbial key players involved in distinct degradation and assimilation processes under in situ conditions. Being dependent on RNA, this technique is particularly suited for environments with high numbers of very active, that is, significantly RNA-expressing microorganisms, such as intestinal tract samples. We use RNA-SIP for the identification of bacteria involved in the degradation and assimilation of prebiotic carbohydrates in order to better understand the functionality of these medically and economically important nutrients in human and animal intestinal environments.
RNA-SIP技术能够将复杂微生物群落的结构与功能联系起来，即识别原位条件下参与不同降解和同化过程的关键微生物。由于该技术依赖RNA，所以特别适用于存在大量高活性（即大量表达RNA）微生物的环境，如肠道样本。我们利用RNA-SIP来鉴定参与益生元碳水化合物降解和同化的细菌，以便更好地了解这些在医学和经济上都很重要的营养素在人和动物肠道环境中的功能。
