肉毒杆菌C2毒素、肉毒杆菌ADP核糖基转移酶C3和百日咳毒素形成的不同类型的ADP-核糖蛋白键。
Different types of ADP-ribose protein bonds formed by botulinum C2 toxin, botulinum ADP-ribosyltransferase C3 and pertussis toxin.
作者信息
Aktories K, Just I, Rosenthal W
机构信息
Rudolf-Buchheim-Institut für Pharmakologie, Justus-Liebig Universität Giessen, F.R.G.
出版信息
Biochem Biophys Res Commun. 1988 Oct 14;156(1):361-7. doi: 10.1016/s0006-291x(88)80849-0.
We attempted to characterize ADP-ribose-amino acid bonds formed by various bacterial toxins. The ADP-ribose-arginine bond formed by botulinum C2 toxin in actin was cleaved with a half-life of about 2 h by treatment with hydroxylamine (0.5 M). In contrast, the ADP-ribose-cysteine bond formed by pertussis toxin in transducin and the ADP-ribose-amino acid linkage formed by botulinum ADP-ribosyltransferase C3 in platelet cytosolic proteins were not affected by hydroxylamine. HgCl2 cleaved the ADP-ribose-amino acid bond formed by pertussis toxin in transducin but not those formed by botulinum C2 toxin or botulinum ADP-ribosyltransferase C3 in actin and platelet cytosolic proteins, respectively. NaOH (0.5 M) cleaved the ADP-ribose-amino acid bonds formed by botulinum C2 toxin and pertussis toxin but not the one formed by botulinum ADP-ribosyltransferase C3. The data indicate that the ADP-ribose bond formed by botulinum ADP-ribosyltransferase C3 differs from those formed by the known bacterial ADP-ribosylating toxins.
我们试图对各种细菌毒素形成的ADP - 核糖 - 氨基酸键进行表征。肉毒杆菌C2毒素在肌动蛋白中形成的ADP - 核糖 - 精氨酸键，用0.5 M羟胺处理后，以约2小时的半衰期被裂解。相比之下，百日咳毒素在转导素中形成的ADP - 核糖 - 半胱氨酸键以及肉毒杆菌ADP - 核糖基转移酶C3在血小板胞质蛋白中形成的ADP - 核糖 - 氨基酸连接不受羟胺影响。HgCl2可裂解百日咳毒素在转导素中形成的ADP - 核糖 - 氨基酸键，但不能裂解肉毒杆菌C2毒素或肉毒杆菌ADP - 核糖基转移酶C3分别在肌动蛋白和血小板胞质蛋白中形成的键。0.5 M NaOH可裂解肉毒杆菌C2毒素和百日咳毒素形成的ADP - 核糖 - 氨基酸键，但不能裂解肉毒杆菌ADP - 核糖基转移酶C3形成的键。数据表明，肉毒杆菌ADP - 核糖基转移酶C3形成的ADP - 核糖键与已知的细菌ADP - 核糖基化毒素形成的键不同。
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