Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research, Center for Environmentally-Friendly Aquaculture, Guangzhou, 510631, PR China.
Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangdong Provincial Engineering Technology Research, Center for Environmentally-Friendly Aquaculture, Guangzhou, 510631, PR China.
Fish Shellfish Immunol. 2019 Oct;93:612-622. doi: 10.1016/j.fsi.2019.08.023. Epub 2019 Aug 10.
In teleost fish, IgM B cells play important roles in innate and adaptive immunity. Different IgM B cells are detected in teleost, named IgM and IgM B cell subsets, according to the distinct expression levels of membrane IgM (mIgM). However, the study on the heterogeneity in IgM B cell subsets remains poorly understood. In this study, the comparative transcriptomic profiles of IgM, IgM and IgM from peripheral blood of Nile tilapia (Oreochromis niloticus) were carried out by using RNA-sequencing technique. A total of 6045 and 5470 differentially expressed genes (DEGs) were detected in IgM and IgM cells, respectively, as compared with IgM lymphocytes, whereas 3835 genes were differentially expressed when IgM compared to IgM cells. Analysis of the KEGG database indicated that the DEGs were enriched in immune system categories and signaling transduction and interaction in IgM vs IgM, IgM vs IgM and IgM vs IgM. Comparatively, in IgM vs IgM, GO enrichment analysis indicated that the DEGs enriched in nucleic acid binding transcription factor activity. Analysis of crucial transcription factors for B cell differentiation indicated that IgM and IgM cell clusters belonged to the different B cell subsets. The data generated in this study may provide insights into understanding the heterogeneity of IgM cells in teleost, and suggest that IgM B cells play a crucial role in innate immunity.
在硬骨鱼类中，IgM B 细胞在先天免疫和适应性免疫中发挥重要作用。根据膜免疫球蛋白 mIgM 的不同表达水平，硬骨鱼类中检测到不同的 IgM B 细胞，分别命名为 IgM 和 IgM B 细胞亚群。然而，IgM B 细胞亚群的异质性研究仍知之甚少。本研究采用 RNA 测序技术，对尼罗罗非鱼（Oreochromis niloticus）外周血中的 IgM、IgM 和 IgM 进行了比较转录组分析。与 IgM 淋巴细胞相比，IgM 和 IgM 细胞分别检测到 6045 和 5470 个差异表达基因（DEGs），而 IgM 与 IgM 细胞相比则有 3835 个基因差异表达。KEGG 数据库分析表明，DEGs 在免疫和信号转导及相互作用类别中富集。与 IgM 相比，IgM 与 IgM 和 IgM 相比，GO 富集分析表明 DEGs 富集在核酸结合转录因子活性。分析 B 细胞分化的关键转录因子表明，IgM 和 IgM 细胞簇属于不同的 B 细胞亚群。本研究产生的数据可能有助于理解硬骨鱼类 IgM 细胞的异质性，并表明 IgM B 细胞在先天免疫中发挥关键作用。
