Miller I F, Mayoral J, Djordjevich L, Kashani A
University of Illinois, Chicago.
Biomater Artif Cells Artif Organs. 1988;16(1-3):281-8. doi: 10.3109/10731198809132577.
The ability of liposome-encapsulated hemoglobin (LEH) to sustain life in rats at hematocrits 45% below lethal levels was demonstrated by virtually total exchange transfusions. All control animals (transfused with 7% albumin in Erilyte) died at a mean hematocrit of 5.35%, with corresponding oxygen-carrying capacity of 2.65 ml/100 ml. All LEH animals survived with a final RBC hematocrit of 2.96% and an oxygen-carrying capacity of 7.05 ml/100 ml. LEH animals maintained normal blood pressures and essentially normal cardiac output, while control animals failed to do so. Systemic vascular resistance of control animals at 78% exchange was reduced to 33% of baseline, while of LEH animals at 92% exchange, to 80% of baseline. In-vitro measurements were made of LEH and RBC suspension viscosity as a function of shear rate, suspension concentration, and lipid membrane composition. The viscous behavior of the LEH suspensions was non-Newtonian and nearly the same as suspensions of natural RBC prepared similarly, though LEH suspensions had slightly higher viscosity.
通过几乎完全的换血，证明了脂质体包裹的血红蛋白（LEH）在大鼠血细胞比容低于致死水平45%时维持生命的能力。所有对照动物（输注含7%白蛋白的电解质溶液）在平均血细胞比容为5.35%时死亡，相应的携氧能力为2.65 ml/100 ml。所有LEH动物存活下来，最终红细胞血细胞比容为2.96%，携氧能力为7.05 ml/100 ml。LEH动物维持正常血压和基本正常的心输出量，而对照动物则不能。对照动物在78%换血时的全身血管阻力降至基线的33%，而LEH动物在92%换血时降至基线的80%。对LEH和红细胞悬液的粘度进行了体外测量，测量其作为剪切速率、悬液浓度和脂质膜组成的函数。LEH悬液的粘性行为是非牛顿的，与以类似方式制备的天然红细胞悬液几乎相同，但LEH悬液的粘度略高。
