National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
University of the Chinese Academy of Sciences, Beijing 100049, China.
Plant Physiol. 2019 Oct;181(2):804-816. doi: 10.1104/pp.19.00509. Epub 2019 Aug 13.
During the legume-rhizobium symbiotic interaction, rhizobial invasion of legumes is primarily mediated by a plant-made tubular invagination called an infection thread (IT). Here, we identify a gene in encoding a Leu-rich repeat receptor-like kinase (LRR-RLK), (), that is induced by Nod factors (NFs) and is involved in IT formation but not nodule organogenesis. A paralog, RINRK2, plays a relatively minor role in infection. is required for full induction of early infection genes, including Nodule Inception (), encoding an essential nodulation transcription factor. displayed an infection-specific expression pattern, and NIN bound to the promoter, inducing its expression. was found to be an atypical kinase localized to the plasma membrane and did not require kinase activity for rhizobial infection. We propose RINRK1 is an infection-specific RLK, which may specifically coordinate output from NF signaling or perceive an unknown signal required for rhizobial infection.
在豆科植物-根瘤菌共生相互作用中，根瘤菌对豆科植物的侵染主要是通过一种植物源性的管状内陷结构来介导，这种结构被称为侵染线（IT）。在这里，我们鉴定了一个编码富含亮氨酸重复受体样激酶（LRR-RLK）的基因 ，（），它被结瘤因子（NFs）诱导，并参与侵染线的形成，但不参与根瘤器官发生。一个旁系同源物 RINRK2 在侵染中起相对次要的作用。 对于早期侵染基因的完全诱导是必需的，包括编码必需的结瘤转录因子的 Nodule Inception（）。 表现出侵染特异性的表达模式，NIN 结合到 启动子上，诱导其表达。 被发现是一种定位于质膜的非典型激酶，并且不需要激酶活性即可进行根瘤菌侵染。我们提出 RINRK1 是一种侵染特异性的 RLK，它可能专门协调 NF 信号的输出，或者感知到根瘤菌侵染所需的未知信号。
