Chen Kefei, Dai Feihu, Li Guangxu, Dong Jirong, Wang Yuhai
Department of Neurosurgery, Wuxi Clinical College of Anhui Medical University, Craniocerebral Injury Cure Center of People's Liberation Army, The 101st Hospital of People's Liberation Army, Wuxi, Jiangsu 214044, P.R. China.
Exp Ther Med. 2019 Sep;18(3):2104-2110. doi: 10.3892/etm.2019.7785. Epub 2019 Jul 17.
The present study aimed to investigate the use of computerized tomography (CT) perfusion for evaluating cerebral hemodynamics following traumatic brain injury (TBI) in rabbits. The animals were randomly assigned into four groups (n=10 animals/group): i) Control, ii) TBI, iii) TBI common decompression and iv) TBI controlled decompression groups. A TBI model was established in rabbits using epidural balloon inflation. In the groups receiving intervention, animals were provided common decompression or controlled decompression treatments. Conventional CT and CT perfusion scanning were performed, with cerebral hemodynamic indices, including regional cerebral blood flow (rCBF), regional cerebral blood volume (rCBV) and mean transit time (MTT) being measured. Blood-brain barrier (BBB) permeability was evaluated using Evans blue staining. Compared with those in the control group, rCBF and rCBV values of the bilateral temporal lobes and basal ganglion in the TBI, TBI + common decompression and TBI + controlled decompression groups were significantly lower, whereas the MTT values were markedly prolonged and Evans blue dye content was greatly increased (P<0.01). Controlled decompression was demonstrated to be more potent than common decompression for preventing TBI-induced decline in rCBF and rCBV values in the bilateral temporal lobes and basal ganglion, as well as reversing TBI-induced extension of MTT in the bilateral temporal lobes (P<0.01 vs. TBI group). However, neither common nor controlled decompression could reduce TBI-induced increase in BBB permeability. In conclusion, these findings indicate that CT perfusion may be used to monitor cerebral hemodynamics following TBI in rabbits. Controlled decompression was deduced to be more potent than common decompression for preventing abnormalities in cerebral hemodynamics after TBI.
本研究旨在探讨计算机断层扫描（CT）灌注成像在评估兔创伤性脑损伤（TBI）后脑血流动力学方面的应用。将动物随机分为四组（每组n = 10只动物）：i）对照组，ii）TBI组，iii）TBI +常规减压组和iv）TBI +控制性减压组。采用硬膜外气囊充气法建立兔TBI模型。在接受干预的组中，对动物进行常规减压或控制性减压治疗。进行常规CT和CT灌注扫描，测量脑血流动力学指标，包括局部脑血流量（rCBF）、局部脑血容量（rCBV）和平均通过时间（MTT）。使用伊文思蓝染色评估血脑屏障（BBB）通透性。与对照组相比，TBI组、TBI +常规减压组和TBI +控制性减压组双侧颞叶和基底神经节的rCBF和rCBV值显著降低，而MTT值明显延长，伊文思蓝染料含量大幅增加（P < 0.01）。结果表明，控制性减压在预防TBI引起的双侧颞叶和基底神经节rCBF和rCBV值下降以及逆转TBI引起的双侧颞叶MTT延长方面比常规减压更有效（与TBI组相比，P < 0.01）。然而，常规减压和控制性减压均不能降低TBI引起的BBB通透性增加。总之，这些发现表明CT灌注成像可用于监测兔TBI后的脑血流动力学。推断控制性减压在预防TBI后脑血流动力学异常方面比常规减压更有效。
