Ito H, Naito C, Hayashi H, Kawamura M
Department of Internal Medicine, Tokyo Teishin Hospital, Japan.
Clin Chem. 1988 Nov;34(11):2224-7.
The correlation between low-density lipoprotein (LDL) receptor activity and concentrations of lipids and apolipoproteins in serum was examined in 12 subjects with heterozygous familial hypercholesterolemia (FH) and in four with non-FH type II hyperlipoproteinemia. Concentrations of high-density lipoprotein cholesterol and of apolipoproteins (apo) A-I, C-II, and C-III were significantly positively correlated with LDL receptor activity, whereas LDL receptor activity was significantly inversely correlated with LDL cholesterol and apo B concentrations, and with apo ratios B/A-I and B/A-II. Neither total serum cholesterol, triglyceride, phospholipid, apo A-I, nor apo E concentrations correlated significantly with LDL receptor activity. Multiple regression analysis, with LDL receptor activity as the dependent variable, revealed concentrations of apo B and apo C-II to be the principal determinant factors. To confirm this, we subsequently calculated the LDL receptor activities before and after administration of CS-514, an inhibitor of hydroxymethylglutaryl-CoA reductase (EC 1.1.1.88), which increases the hepatic LDL receptor activity and decreases the concentration of cholesterol in serum. This drug increased calculated LDL receptor activities significantly, with a significant decrease in serum cholesterol.
在12名杂合子家族性高胆固醇血症（FH）患者和4名非FH型II型高脂蛋白血症患者中，研究了低密度脂蛋白（LDL）受体活性与血清脂质和载脂蛋白浓度之间的相关性。高密度脂蛋白胆固醇以及载脂蛋白（apo）A-I、C-II和C-III的浓度与LDL受体活性显著正相关，而LDL受体活性与LDL胆固醇和apo B浓度以及apo比值B/A-I和B/A-II显著负相关。血清总胆固醇、甘油三酯、磷脂、apo A-I和apo E浓度均与LDL受体活性无显著相关性。以LDL受体活性为因变量的多元回归分析显示，apo B和apo C-II的浓度是主要决定因素。为证实这一点，我们随后计算了给予CS-514（一种羟甲基戊二酰辅酶A还原酶（EC 1.1.1.88）抑制剂，可增加肝脏LDL受体活性并降低血清胆固醇浓度）前后的LDL受体活性。该药物显著增加了计算得出的LDL受体活性，同时血清胆固醇显著降低。
