Dearakhshandeh Nooshin, Mogheiseh Asghar, Nazifi Saeed, Ahrari Khafi Mohammad Saeed, Abbaszadeh Hasiri Mohammad, Golchin-Rad Kamran
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Fars, Iran.
J Vet Pharmacol Ther. 2019 Nov;42(6):665-672. doi: 10.1111/jvp.12805. Epub 2019 Aug 13.
Finding a medical treatment which can combat cell proliferation and relax smooth muscles in canine benign prostatic hyperplasia (BPH) appears to be imperative.
This study aimed to evaluate the oxidative stress and inflammatory proteins following the treatment of dogs induced for BPH with an anti-proliferative agent called tadalafil.
Twenty-five adult intact male dogs were randomly designated into five groups (n = 5): Control group was not induced for BPH and not treated with tadalafil; dogs induced for BPH by testosterone enanthate and estradiol benzoate and treated with tadalafil (5 mg/day P.O.); dogs which received tadalafil (5 mg/day P.O.); dogs induced for BPH and treated with castration; and dogs induced for BPH. Oxidative stress factors (glutathione peroxidase [GPX], superoxide dismutase [SOD], catalase) and inflammatory proteins (haptoglobin, serum amyloid A [SAA], malondialdehyde [MDA]) were measured in the blood serum for four sequential weeks.
Glutathione peroxidase and SOD serum levels declined in dogs in the BPH-induced group compared to those in the control group. Those levels diminished in BPH-induced castrated and tadalafil-treated groups. The changes in the GPX and SOD serum concentrations were not significant between the BPH-induced castrated group and BPH-induced tadalafil-treated group. Moreover, MDA concentration increased slightly in groups with BPH and groups which were castrated. Generally, however, there were no significant differences in the MDA serum concentrations between other groups. Haptoglobin and SAA concentrations increased in BPH-castrated group. Also, the differences in haptoglobin and SAA were not significant between the groups.
Tadalafil could not control oxidative stress and inflammatory mediators which happened during BPH in dogs.
找到一种能够对抗犬良性前列腺增生（BPH）中细胞增殖并舒张平滑肌的医学治疗方法似乎势在必行。
本研究旨在评估用一种名为他达拉非的抗增殖药物治疗诱导BPH的犬后其氧化应激和炎症蛋白情况。
25只成年未阉割雄性犬被随机分为五组（n = 5）：对照组未诱导BPH且未用他达拉非治疗；用庚酸睾酮和苯甲酸雌二醇诱导BPH并用他达拉非治疗的犬（口服5毫克/天）；接受他达拉非治疗的犬（口服5毫克/天）；诱导BPH并接受去势治疗的犬；以及诱导BPH的犬。连续四周测定血清中的氧化应激因子（谷胱甘肽过氧化物酶[GPX]、超氧化物歧化酶[SOD]、过氧化氢酶）和炎症蛋白（触珠蛋白、血清淀粉样蛋白A[SAA]、丙二醛[MDA]）。
与对照组相比，BPH诱导组犬的血清谷胱甘肽过氧化物酶和SOD水平下降。在BPH诱导的去势组和他达拉非治疗组中这些水平降低。BPH诱导的去势组和BPH诱导的他达拉非治疗组之间，GPX和SOD血清浓度变化不显著。此外，BPH组和去势组的MDA浓度略有增加。然而，总体而言，其他组之间MDA血清浓度无显著差异。BPH去势组的触珠蛋白和SAA浓度增加。而且，各组之间触珠蛋白和SAA的差异不显著。
他达拉非无法控制犬BPH期间发生的氧化应激和炎症介质。
