评估用于从儿童粪便样本中鉴定钩虫种类的各种培养技术。

Evaluation of various culture techniques for identification of hookworm species from stool samples of children.

作者信息

Nongmaithem Onila, Shantikumar T, Dutta Sudip

机构信息

Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Gangtok, India.

Department of Pediatrics, Sikkim Manipal Institute of Medical Sciences, Gangtok, India.

出版信息

Indian J Med Res. 2019 Apr;149(4):539-543. doi: 10.4103/ijmr.IJMR_938_17.

DOI:10.4103/ijmr.IJMR_938_17

PMID:31411178

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6676850/

Abstract

BACKGROUND & OBJECTIVES: Different coproculture techniques have been developed for culturing the hookworm (HW) larvae for morphological identification in the resource-limited settings. The objective of this study was to compare the performances of Harada-Mori culture (HMC), agar plate culture (APC) and modified APC (MAPC) of HW positive stool specimens for identification of HW species in East Sikkim.

METHODS

This prospective study was done in East Sikkim from May 2015 to May 2016. Stool and blood samples were collected from paediatric patients with gastrointestinal symptoms. The HW positive stool specimens by microscopy were subjected to HMC, APC and MAPC techniques to harvest HW larvae. Stoll's dilution egg count for determining egg intensity and blood parameters were performed in all the 12 HW-positive patients.

RESULTS

Twelve of the 180 samples were found positive for HW by microscopy and predominance of Necator americanus (75%) over Ancylostoma duodenale (25%) was observed. Blood parameters results showed high pack cell volume (PCV) values in 78.6 per cent, anaemia in 75 per cent and high eosinophil count in most patients. Stoll's dilution egg count showed moderate infection in 66.6 per cent, light and heavy infections in 16.7 per cent each.

INTERPRETATION & CONCLUSIONS: Our results showed that APC yielded 100 per cent results and was easier to perform in the laboratory compared to MAPC and HMC techniques.

摘要

背景与目的

为在资源有限的环境中培养钩虫幼虫以进行形态学鉴定，已开发出不同的共培养技术。本研究的目的是比较在东锡金邦对钩虫阳性粪便标本进行的原田-森氏培养（HMC）、琼脂平板培养（APC）和改良琼脂平板培养（MAPC）在钩虫种类鉴定方面的性能。

方法

本前瞻性研究于2015年5月至2016年5月在东锡金邦进行。从有胃肠道症状的儿科患者中采集粪便和血液样本。通过显微镜检查为钩虫阳性的粪便标本采用HMC、APC和MAPC技术来收集钩虫幼虫。对所有12名钩虫阳性患者进行了用于确定虫卵强度的司徒尔氏稀释虫卵计数和血液参数检测。

结果

180份样本中有12份经显微镜检查发现钩虫阳性，观察到美洲板口线虫占优势（75%），十二指肠钩口线虫占25%。血液参数结果显示，78.6%的患者血细胞比容（PCV）值高，75%的患者贫血，大多数患者嗜酸性粒细胞计数高。司徒尔氏稀释虫卵计数显示，66.6%的患者为中度感染，16.7%的患者为轻度和重度感染。