Zuber P, Accolla R S, Carrel S, Diserens A C, de Tribolet N
Department of Neurosurgery, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
Int J Cancer. 1988 Nov 15;42(5):780-6. doi: 10.1002/ijc.2910420525.
Human malignant glioma cell lines and clones were incubated with various concentrations of recombinant human TNF-alpha, either alone or in combination with recombinant human IFN-gamma. The surface expression of HLA-ABC (class I) antigens and beta 2-microglobulin, was significantly enhanced by TNF-alpha alone on every cell line and clone tested. After incubation with both TNF-alpha and IFN-gamma, the surface expression of HLA-ABC antigens was only slightly higher than that observed with each cytokine alone. In contrast to IFN-gamma, TNF-alpha had no effect on the surface expression of HLA-DR (class II) antigens. Moreover, the surface expression of HLA-DR induced by IFN-gamma was unaffected by TNF-alpha. The increased expression of HLA-ABC antigens after treatment with TNF-alpha or IFN-gamma correlated with increased levels of HLA-ABC-specific mRNA. In addition, TNF-alpha, like IFN-gamma, selectively enhanced the surface expression of a tumor-associated antigen, Me14-D12, while it had no effect on the expression of various other surface antigens. In the absence of actinomycin D, TNF-alpha exhibited no direct cytotoxic/cytostatic effect on the glioma cell lines tested. These results indicate that TNF-alpha can enhance the surface expression of HLA-ABC antigens on human glioma cells in the absence of a direct cytotoxic/cytostatic effect.
将人恶性胶质瘤细胞系和克隆体与不同浓度的重组人肿瘤坏死因子-α（TNF-α）单独或与重组人干扰素-γ（IFN-γ）联合孵育。单独使用TNF-α时，在每个测试的细胞系和克隆体上，HLA-ABC（I类）抗原和β2-微球蛋白的表面表达均显著增强。在用TNF-α和IFN-γ共同孵育后，HLA-ABC抗原的表面表达仅略高于单独使用每种细胞因子时观察到的表达水平。与IFN-γ相反，TNF-α对HLA-DR（II类）抗原的表面表达没有影响。此外，IFN-γ诱导的HLA-DR表面表达不受TNF-α的影响。用TNF-α或IFN-γ处理后HLA-ABC抗原表达的增加与HLA-ABC特异性mRNA水平的增加相关。此外，TNF-α与IFN-γ一样，选择性地增强了肿瘤相关抗原Me14-D12的表面表达，而对其他各种表面抗原的表达没有影响。在没有放线菌素D的情况下，TNF-α对所测试的胶质瘤细胞系没有直接的细胞毒性/细胞抑制作用。这些结果表明，在没有直接细胞毒性/细胞抑制作用的情况下，TNF-α可以增强人胶质瘤细胞上HLA-ABC抗原的表面表达。
