大肠杆菌稳定期细胞中的肽聚糖生物合成
Peptidoglycan biosynthesis in stationary-phase cells of Escherichia coli.
作者信息
Blasco B, Pisabarro A G, de Pedro M A
机构信息
Instituto de Biologia Molecular, Facultad de Ciencias, Universidad Autonoma de Madrid, Spain.
出版信息
J Bacteriol. 1988 Nov;170(11):5224-8. doi: 10.1128/jb.170.11.5224-5228.1988.
Abstract
The ability of stationary-phase cells of Escherichia coli W7 to incorporate radioactive precursors into macromolecular murein has been studied. During the initial 6 h of the stationary phase, resting cells incorporated meso-[3H]diaminopimelic acid at a rate corresponding to the insertion of 1.3 X 10(4) disaccharide units min-1 cell-1. Afterwards, the rate of incorporation dropped drastically (90%) to a low but still detectable level. Incorporation during stationary phase did not result in an increased amount of total murein in the culture, suggesting that it was related to a turnover process. Analysis of the effects of a number of beta-lactam antibiotics indicated that incorporation of murein precursors in stationary-phase cells was mediated by penicillin-binding proteins, suggesting that the activity of penicillin-binding protein 2 was particularly relevant to this process.
摘要
对大肠杆菌W7稳定期细胞将放射性前体掺入大分子胞壁质的能力进行了研究。在稳定期的最初6小时内，静止细胞以每分钟每细胞插入1.3×10⁴个二糖单位的速率掺入内消旋-[³H]二氨基庚二酸。此后，掺入速率急剧下降（90%）至一个低但仍可检测到的水平。稳定期的掺入并未导致培养物中总胞壁质数量增加，这表明其与周转过程有关。对多种β-内酰胺抗生素作用的分析表明，稳定期细胞中胞壁质前体的掺入是由青霉素结合蛋白介导的，这表明青霉素结合蛋白2的活性与该过程特别相关。
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