Albrecht D L, Noelle R J
Biochemistry Graduate Program, Dartmouth Medical School, Hanover, NH 03756.
J Immunol. 1988 Dec 1;141(11):3915-22.
Membrane IgM (mIgM) and mIgD are the receptors for Ag on the surface of B lymphocytes, mIg is soluble in detergent; however, when mIg is cross-linked with anti-Ig, the mIg becomes associated with the cytoskeletal matrix and is rendered detergent-insoluble. By a novel flow cytofluorometric assay and by biochemical analysis, it has been shown that anti-isotype-specific antibodies induce mIgM and mIgD to associate with the cytoskeleton of B lymphocytes in an isotype-specific fashion. The detergent solubility of other prominent B lymphocyte surface proteins, such as class I and class II MHC proteins were unaffected by cross-linking of mIg. A panel of mu-specific mAb was analyzed for their ability to induce mIgM-cytoskeletal association. Although all mAb bound mIgM, only three out of seven rendered mIgM cytoskeletally associated. Further analysis revealed a strict correlation in the capacity of mu-specific mAb to induce capping and to induce the association of mIgM with the cytoskeleton.
膜免疫球蛋白M（mIgM）和mIgD是B淋巴细胞表面的抗原受体，mIg可溶于去污剂；然而，当mIg与抗Ig交联时，mIg会与细胞骨架基质结合并变得不溶于去污剂。通过一种新型的流式细胞荧光测定法和生化分析表明，抗同种型特异性抗体以同种型特异性方式诱导mIgM和mIgD与B淋巴细胞的细胞骨架结合。其他重要的B淋巴细胞表面蛋白，如I类和II类主要组织相容性复合体蛋白的去污剂溶解性不受mIg交联的影响。分析了一组μ特异性单克隆抗体诱导mIgM-细胞骨架结合的能力。尽管所有单克隆抗体都能结合mIgM，但七分之三只使mIgM与细胞骨架结合。进一步分析表明，μ特异性单克隆抗体诱导帽化和诱导mIgM与细胞骨架结合的能力之间存在严格的相关性。
