Nakibirango Juliet, Mugenyi Vincent, Nsaba Dickson, Nsimemukama Aron, Rugera Simon Peter, Okongo Benson
Department of Medical Laboratory Science, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda.
HIV AIDS (Auckl). 2019 Jun 18;11:141-145. doi: 10.2147/HIV.S206195. eCollection 2019.
To determine the prevalence of by age, sex, and duration on antiretroviral therapy (ART) and establish hygienic malpractices that may predispose to infection. We enrolled 138 HIV/AIDS patients on ART from June to October 2018. Stool samples were collected from study participants, wet saline preparations made and examined, stool samples concentrated using formal ether concentration, and smears stained using the modified Ziehl-Neelsen technique. Structured questionnaires were used to collect demographic data and hygienic malpractices that predisposed study participants to cryptosporidiosis infection. Of 138, 99 (71.7%) were females and 39 (28.7%) males. The age range was 9-69 years and mean age 37 years. The overall prevalence of cryptosporidiosis was three (2.17%). The most affected age-groups were 31-40 years (3.85%) and 21-30 years (3.22%), and only females (3.03%) were affected. The distribution of cryptosporidiosis according to the duration spent on ART showed that those who had spent <1 year on ART were the most affected (11.1%), followed by those who had spent 1-5 years 1 (2.2%), while those patients on ART for 6-10 years were 1 (1.7%) and those on ART for more than 10 years were not affected. There was no significant association between cryptosporidiosis and sex (=0.272), educational background (=0.670), handwashing (=0.853), drinking boiled water (=0.818), duration on ART (=0.263), occupation (=0.836), and age (=0.723). The prevalence reported in this study is low; however, it is still vital for clinicians to proceed to have cryptosporidiosis as the main differential in HIV/AIDS patients with gastrointestinal infections.
按年龄、性别和抗逆转录病毒疗法(ART)疗程确定隐孢子虫病的患病率,并确定可能易引发感染的卫生习惯不良情况。我们纳入了2018年6月至10月期间138例接受ART治疗的艾滋病毒/艾滋病患者。从研究参与者中采集粪便样本,制作湿盐水涂片并进行检查,使用甲醛乙醚浓缩法对粪便样本进行浓缩,并用改良齐尔-尼尔森技术对涂片进行染色。使用结构化问卷收集人口统计学数据以及使研究参与者易患隐孢子虫病感染的卫生习惯不良情况。138例患者中,99例(71.7%)为女性,39例(28.7%)为男性。年龄范围为9至69岁,平均年龄37岁。隐孢子虫病的总体患病率为3例(2.17%)。受影响最严重的年龄组为31至40岁(3.85%)和21至30岁(3.22%),且仅女性(3.03%)受到影响。根据ART疗程时长对隐孢子虫病分布情况的分析显示,接受ART治疗时间<1年的患者受影响最大(11.1%),其次是接受ART治疗1至5年的患者(2.2%),而接受ART治疗6至10年的患者中有1例(1.7%),接受ART治疗超过10年的患者未受影响。隐孢子虫病与性别(P=0.272)、教育背景(P=0.670)、洗手(P=0.853)、饮用开水(P=0.818)、ART疗程时长(P=0.263)、职业(P=0.836)和年龄(P=0.723)之间均无显著关联。本研究报告的患病率较低;然而,对于临床医生而言,在患有胃肠道感染的艾滋病毒/艾滋病患者中将隐孢子虫病作为主要鉴别诊断疾病仍然至关重要。