Dusenbery R L, Lee-Chen S F
Department of Biological Sciences, Dedman College, Southern Methodist University, Dallas, TX 75275.
Mutat Res. 1988 Nov;194(3):257-61. doi: 10.1016/0167-8817(88)90027-2.
Autoradiographic analysis of unscheduled DNA synthesis in response to DNA damage produced by relatively high doses of the direct-acting, monofunctional alkylating agent, MMS, (4.5 mM) and UV (40 J/m2) demonstrates that established cell lines, derived from the mei-9a and mus201D1 excision repair-deficient strains of Drosophila melanogaster, perform no measurable incorporation of [3H]thymidine into repair patches, in accordance with the observations made for the corresponding primary embryonic cells derived from the two mutagen-sensitive strains of flies. These established cell lines can therefore be used as appropriate models for both the examination of the biochemical basis of the genetic defects in the mei-9 and mus201 mutations and the role of excision-repair processes in spontaneous and induced mutation induction in eukaryotic cells.
对由相对高剂量的直接作用单功能烷基化剂甲磺酸甲酯（MMS，4.5 mM）和紫外线（40 J/m²）产生的DNA损伤所引发的非预定DNA合成进行放射自显影分析表明，源自黑腹果蝇mei-9a和mus201D1切除修复缺陷菌株的已建立细胞系，不会将[³H]胸腺嘧啶核苷显著掺入修复斑块中，这与对源自这两种诱变敏感果蝇菌株的相应原代胚胎细胞的观察结果一致。因此，这些已建立的细胞系可作为合适的模型，用于研究mei-9和mus201突变中遗传缺陷的生化基础，以及切除修复过程在真核细胞自发和诱导突变诱导中的作用。
