Medical Faculty, Department of Physiology, Selcuk University, Konya, Turkey.
Department of Physiology, Meram Medical School, Necmettin Erbakan University, Konya, Turkey.
J Mol Neurosci. 2019 Dec;69(4):636-642. doi: 10.1007/s12031-019-01392-y. Epub 2019 Aug 15.
Melatonin is a neurohormone that has anticonvulsant activity in different experimental seizure models including hyperthermic febrile seizure. However, the mechanisms of this effect are not clear at the receptor level. The aim of the study was to determine which melatonin receptors involve in the hyperthermic febrile seizure model. 22-30 days Wistar male rats were used, and in children, it corresponds to 1.5-2 years. Groups were performed as (1) control, (2) ethanol/saline, (3) DMSO, (4) melatonin (MT), (5) MT + luzindole (LUZ), (6) MT + K-185, (7) MT + prazosin (PRZ), (8) MT + LUZ + K-185, (9) MT + LUZ + PRZ, (10) MT + K-185 + PRZ, and (11) MT + LUZ + PRZ + K-185. The hyperthermic febrile seizure pattern was established by keeping the rats in 45 °C hot water, and the latency, duration, and severity of seizures were determined in all groups. MT, LUZ, K-185, and PRZ were given 15, 45, 15, and 30 min before the induction of seizure, respectively. It was observed that melatonin shortened the duration of seizure, reduced the severity, and did not affect latency and that these effects were not completely blocked by receptor antagonists when compared with control, ethanol/saline, and DMSO groups. In conclusion, the fact that the anticonvulsant effect of melatonin is not completely blocked by all melatonin receptor antagonists. We can conclude that a multimodal mechanism may be responsible for the effect of melatonin receptors alone on the anticonvulsant effect of melatonin. It will be useful to design new pharmacological studies to make the subject clear.
褪黑素是一种神经激素，在包括高热性惊厥在内的不同实验性癫痫模型中具有抗惊厥活性。然而，其受体水平的作用机制尚不清楚。本研究旨在确定哪些褪黑素受体参与高热性惊厥模型。使用 22-30 天龄雄性 Wistar 大鼠，在儿童中相当于 1.5-2 岁。各组分别为（1）对照组，（2）乙醇/盐水，（3）DMSO，（4）褪黑素（MT），（5）MT+Luzindole（LUZ），（6）MT+K-185，（7）MT+Prazosin（PRZ），（8）MT+LUZ+K-185，（9）MT+LUZ+PRZ，（10）MT+K-185+PRZ，和（11）MT+LUZ+PRZ+K-185。通过将大鼠置于 45°C 热水中建立高热性惊厥模式，并在所有组中确定惊厥的潜伏期、持续时间和严重程度。MT、LUZ、K-185 和 PRZ 分别在惊厥诱导前 15、45、15 和 30 分钟给药。结果观察到，褪黑素缩短了惊厥的持续时间，降低了严重程度，且对潜伏期无影响，与对照组、乙醇/盐水和 DMSO 组相比，这些作用并未被受体拮抗剂完全阻断。总之，褪黑素的抗惊厥作用不能被所有褪黑素受体拮抗剂完全阻断。我们可以得出结论，一种多模式机制可能是褪黑素受体单独作用于褪黑素抗惊厥作用的原因。这将有助于设计新的药理学研究，以使该主题更加清晰。
