脂氧合酶的氢过氧化物酶活性：在亚油酸存在下外源物质代谢的潜在途径。

Hydroperoxidase activity of lipoxygenase: a potential pathway for xenobiotic metabolism in the presence of linoleic acid.

作者信息

Kulkarni A P, Cook D C

机构信息

Florida Toxicology Research Center, College of Public Health, University of South Florida, Tampa 33612.

出版信息

Res Commun Chem Pathol Pharmacol. 1988 Sep;61(3):305-14.

PMID:3141996

Abstract

The ability of hydroperoxidase activity of soybean lipoxygenase to co-oxidize xenobiotics was examined in the presence of linoleic acid. The guaiacol oxidation rate was proportional to the linoleic acid dioxygenation rate of the enzyme preparation. The tetraguaiacol formation was dependent upon the concentration of guaiacol, linoleic acid and the amount of enzyme. Boiled enzyme was devoid of activity suggesting enzymatic nature of the co-oxidation reaction. Besides guaiacol, benzidine, tetramethyl-p-phenylenediamine, dimethoxybenzidine, 2,2'-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid), p-phenylenediamine and tetramethylbenzidine were oxidized suggesting that the hydroperoxidase activity of lipoxygenase may be a potential pathway of xenobiotic oxidation.

摘要

在亚油酸存在的情况下，研究了大豆脂氧合酶的氢过氧化物酶活性对异生物质进行共氧化的能力。愈创木酚的氧化速率与酶制剂中亚油酸的双加氧速率成正比。四愈创木酚的形成取决于愈创木酚的浓度、亚油酸和酶的量。煮沸的酶没有活性，这表明共氧化反应具有酶促性质。除了愈创木酚外，联苯胺、四甲基对苯二胺、二甲氧基联苯胺、2,2'-偶氮双(3-乙基苯并噻唑啉-6-磺酸)、对苯二胺和四甲基联苯胺也被氧化，这表明脂氧合酶的氢过氧化物酶活性可能是异生物质氧化的潜在途径。