Walaas S I, Jahn R, Greengard P
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York 10021.
Synapse. 1988;2(5):516-20. doi: 10.1002/syn.890020507.
This study has assessed the contributions of the corticostriatal fibers, the ascending striatopetal fibers, and the intrinsic neostriatal neurons to the nerve terminal population found in the rat neostriatum (caudatoputamen). For this purpose, we have analysed the levels of two different synaptic vesicle-associated proteins, synapsin I and protein p38 (also called synaptophysin), in the neostriatum after specific lesions. Our results indicate that 45-50% of the synaptic vesicle proteins in the rat neostriatum derive from the corticostriatal fibers, that approximately 25-30% of the synaptic vesicle proteins are present in kainic acid-sensitive structures, presumably intrinsic terminals and local collaterals, and that ascending fibers contain 20-25% of the vesicle-associated proteins in the neostriatum. These three neuronal populations therefore comprise 95-100% of the synaptic vesicle-associated proteins in the rat neostriatum, and thus make up most of the nerve terminals in this brain region. The results, which are in general agreement with previous morphometric studies on the rat basal ganglia, therefore indicate that nerve terminals in the central nervous system can be quantitated by use of these biochemical nerve terminal markers. The results also indicate that a somewhat higher percentage of neostriatal nerve terminals belongs to the corticostriatal fibers that previously believed.
本研究评估了皮质纹状体纤维、上升性纹状体向心性纤维和新纹状体内在神经元对大鼠新纹状体（尾状壳核）中神经末梢群体的贡献。为此，我们分析了特定损伤后新纹状体中两种不同的突触囊泡相关蛋白——突触素I和蛋白p38（也称为突触小泡蛋白）的水平。我们的结果表明，大鼠新纹状体中45 - 50%的突触囊泡蛋白来自皮质纹状体纤维，约25 - 30%的突触囊泡蛋白存在于对 kainic 酸敏感的结构中，推测为内在终末和局部侧支，并且上升性纤维含有新纹状体中20 - 25%的囊泡相关蛋白。因此，这三个神经元群体占大鼠新纹状体中突触囊泡相关蛋白的95 - 100%，从而构成了该脑区大部分的神经末梢。这些结果总体上与先前对大鼠基底神经节的形态学研究一致，因此表明中枢神经系统中的神经末梢可以通过使用这些生化神经末梢标记物进行定量。结果还表明，新纹状体神经末梢中属于皮质纹状体纤维的比例比先前认为的要略高一些。
