Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People's Republic of China.
Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, People's Republic of China.
Mol Cell Endocrinol. 2019 Dec 1;498:110540. doi: 10.1016/j.mce.2019.110540. Epub 2019 Aug 14.
Polycystic ovary syndrome (PCOS) is the most common cause of anovulatory infertility in reproductive-aged women; however, its etiology remains poorly understood. This study aimed to reveal the role of miR-29a in PCOS. MiR-29a levels were measured in the granulosa cells (GCs) of forty-seven PCOS patients and forty-seven controls. A receiver operating characteristic (ROC) curve was drawn to evaluate the diagnostic value of miR-29a in non-hyperandrogenism PCOS. MiR-29a was overexpressed in KGN and COV434 cells to examine its roles in proliferation, cell-cycle progression, and steroidogenesis. MiR-29a was significantly down-regulated in PCOS patients, and associated with an increased antral follicle count. The ROC curve showed a major separation between PCOS patients and controls. MiR-29a overexpression in KGN and COV434 cells inhibited cell proliferation, arrested cell-cycle progression, and decreased aromatase expression and estradiol production. These findings suggest that miR-29a is involved in GC proliferation and steroidogenesis, providing insights into PCOS pathogenesis.
多囊卵巢综合征（PCOS）是育龄妇女排卵障碍性不孕的最常见原因；然而，其病因仍知之甚少。本研究旨在揭示 miR-29a 在 PCOS 中的作用。测量了 47 例 PCOS 患者和 47 例对照者的颗粒细胞（GC）中的 miR-29a 水平。绘制受试者工作特征（ROC）曲线以评估 miR-29a 在非高雄激素血症 PCOS 中的诊断价值。在 KGN 和 COV434 细胞中过表达 miR-29a 以检查其在增殖、细胞周期进展和类固醇生成中的作用。PCOS 患者中 miR-29a 显著下调，与窦卵泡计数增加相关。ROC 曲线显示 PCOS 患者和对照组之间存在明显分离。KGN 和 COV434 细胞中 miR-29a 的过表达抑制细胞增殖，阻滞细胞周期进展，并降低芳香化酶表达和雌二醇生成。这些发现表明 miR-29a 参与 GC 增殖和类固醇生成，为 PCOS 发病机制提供了新的见解。
