重组酵母分泌细菌菊粉蔗糖酶发酵生产中链果聚糖
Microbial production of medium chain fructooligosaccharides by recombinant yeast secreting bacterial inulosucrase.
机构信息
Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.
Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
出版信息
Enzyme Microb Technol. 2019 Nov;130:109364. doi: 10.1016/j.enzmictec.2019.109364. Epub 2019 Jun 18.
A high yielding and straightforward production system of fructooligosaccharide (FOS) was developed for industrial production of prebiotics. To increase conversion yield of FOS from sucrose, recombinant yeast secreting inulosucrase from Lactobacillus reuteri (LrInu) were constructed. Efficient secretion of LrInu was achieved by truncation of both amino- and carboxy-termini (LrInuΔNC) and by introducing an optimal secretion signal. The recombinant yeast produced 220 U/mL of recombinant LrInuΔNC into culture medium during fed-batch fermentation. By direct fermentation of recombinant yeast in medium containing sucrose, 128.4 g/L of FOS was produced with 85.6% conversion yield from 300 g/L sucrose, and the highest titer was 152.6 g/L from 400 g/L sucrose. The degree of polymerization of generated FOS was 2-20 indicating medium chain (mcFOS) range. This is the first report of industrially applicable production of mcFOS by recombinant yeast secreting bacterial inulosucrase.
为了工业生产益生元，开发了一种高产且简单的低聚果糖（FOS）生产系统。为了提高蔗糖转化为 FOS 的产率，构建了分泌来自鼠李糖乳杆菌（Lactobacillus reuteri）的纤维二糖酶（LrInu）的重组酵母。通过截短氨基和羧基末端（LrInuΔNC）并引入最佳分泌信号，实现了 LrInu 的有效分泌。在分批补料发酵过程中，重组酵母将 220 U/mL 的重组 LrInuΔNC 分泌到培养基中。通过在含有蔗糖的培养基中直接发酵重组酵母，从 300 g/L 的蔗糖中以 85.6%的转化率生产了 128.4 g/L 的 FOS，最高产量为 400 g/L 蔗糖时为 152.6 g/L。生成的 FOS 的聚合度为 2-20，表明为中链（mcFOS）范围。这是首例通过分泌细菌纤维二糖酶的重组酵母工业生产 mcFOS 的报道。
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