在水杨酸存在下，紫外光诱导的胸腺嘧啶和抗坏血酸的反应。

Reaction of thymidine and ascorbic acid induced by UV in the presence of salicylic acid.

机构信息

School of Pharmacy, Shujitsu University, 1-6-1 Nishigawara, Okayama 703-8516, Japan.

出版信息

Bioorg Med Chem. 2019 Oct 1;27(19):115046. doi: 10.1016/j.bmc.2019.115046. Epub 2019 Aug 9.

DOI:10.1016/j.bmc.2019.115046

PMID:31422010

Abstract

When a neutral solution of thymidine and ascorbic acid was irradiated with UV light of wavelength longer than 300 nm in the presence of salicylic acid as a photosensitizer, six product peaks appeared in an HPLC chromatogram in addition to small amounts of thymidine dimers. The six products were identified as three pairs of diastereomers of 5-(2-deoxy-2-l-ascorbyl)-5,6-dihydrothymidine, 5-(2-l-ascorbyl)-5,6-dihydrothymidine, and 5,6-dihydrothymidine. These results suggest that novel DNA damage may be generated by ascorbic acid with salicylic acid induced by sunlight.

摘要

当含有抗坏血酸的胸苷中性溶液在水杨酸作为光敏剂存在的情况下，用波长大于 300nm 的紫外光照射时，除了少量的胸苷二聚体外，HPLC 色谱图中还出现了六个产物峰。这六种产物被鉴定为 5-(2-脱氧-2-L-抗坏血酸)-5,6-二氢胸苷、5-(2-L-抗坏血酸)-5,6-二氢胸苷和 5,6-二氢胸苷的三对非对映异构体。这些结果表明，新型 DNA 损伤可能是由阳光诱导的抗坏血酸和水杨酸产生的。