Ryan White Center for Pediatric Infectious Diseases and Global Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Ryan White Center for Pediatric Infectious Diseases and Global Health, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Diagn Microbiol Infect Dis. 2019 Nov;95(3):114841. doi: 10.1016/j.diagmicrobio.2019.05.013. Epub 2019 May 29.
To compare the performance and time-to-result (TTR) for antimicrobial susceptibility testing (AST) of positive blood cultures (PBC) using the Accelerate Pheno™ system (AXDX) and both a direct VITEK® 2 card inoculation workflow (DV2) and traditional FDA-approved VITEK® 2 workflow using subcultured isolates (V2).
Patient samples with monomicrobial Gram-negative rod bacteremia were tested on AXDX and DV2 in tandem and compared to V2 AST results. Categorical agreement (CA) errors were adjudicated using broth microdilution. Instrumentation times and AST TTR were compared.
AXDX and DV2 had a CA of 93.4% and 97.4%, respectively, compared to V2. Postadjudication, AXDX, DV2, and V2 had CA of 94.7%, 95.7%, and 96.5%, respectively. Instrument run times were 6.6 h, 9.4 h, and 9.2 h, and AST TTR were 8.9 h, 12.9 h and 35.5 h, respectively.
AXDX and DV2 ASTs are fast and reliable, which may have significant antimicrobial stewardship implications.
比较使用 Accelerate Pheno™ 系统（AXDX）与直接 VITEK® 2 卡接种工作流程（DV2）和使用分离培养物的传统 FDA 批准的 VITEK® 2 工作流程（V2）对阳性血培养物（PBC）进行抗菌药物敏感性测试（AST）的性能和结果时间（TTR）。
对 AXDX 和 DV2 进行串联检测，以检测革兰氏阴性棒状菌单微生物菌血症患者样本，并与 V2 AST 结果进行比较。使用肉汤微量稀释法对分类一致性（CA）错误进行裁决。比较仪器时间和 AST TTR。
AXDX 和 DV2 与 V2 相比，CA 分别为 93.4%和 97.4%。经裁决后，AXDX、DV2 和 V2 的 CA 分别为 94.7%、95.7%和 96.5%。仪器运行时间分别为 6.6 小时、9.4 小时和 9.2 小时，AST TTR 分别为 8.9 小时、12.9 小时和 35.5 小时。
AXDX 和 DV2 的 AST 快速且可靠，这可能对抗菌药物管理有重大意义。
