催乳素对细菌毒素诱导的Nb2淋巴瘤细胞膜蛋白的ADP核糖基化有不同影响。

Prolactin differentially affects bacterial toxin-induced ADP-ribosylation of Nb2 lymphoma cell membrane proteins.

作者信息

Barkey R J, Calvo J C, Dufau M L

机构信息

Molecular Endocrinology Section, National Institutes of Child Health and Human Development, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1988 Oct 31;156(2):776-82. doi: 10.1016/s0006-291x(88)80911-2.

DOI:10.1016/s0006-291x(88)80911-2

PMID:3142463

Abstract

Exposure of lactogen-dependent Nb2 lymphoma cells to prolactin for up to 72 hr caused time- and dose-dependent changes in the ability of specific 38 kDa and 41.5 kDa membrane proteins from these cells to be subsequently ADP-ribosylated by pertussis and cholera toxins, respectively. Whereas the sensitivity of the 41.5 kDa substrate to cholera toxin was already reduced after 1 hour, that of the 38 kDa substrate for cholera toxin was increased for up to 72 hours. These findings suggest that membrane G-proteins may mediate the effects of prolactin binding to its receptor, leading to the proliferation of Nb2 lymphoma cells.

摘要

将依赖催乳素的Nb2淋巴瘤细胞暴露于催乳素长达72小时，会导致这些细胞中特定的38 kDa和41.5 kDa膜蛋白随后分别被百日咳毒素和霍乱毒素进行ADP核糖基化的能力发生时间和剂量依赖性变化。41.5 kDa底物对霍乱毒素的敏感性在1小时后就已降低，而38 kDa底物对霍乱毒素的敏感性则在长达72小时内增加。这些发现表明，膜G蛋白可能介导催乳素与其受体结合的效应，从而导致Nb2淋巴瘤细胞的增殖。