高等植物质膜中GTP结合蛋白的鉴定
Identification of GTP-binding proteins in the plasma membrane of higher plants.
作者信息
Blum W, Hinsch K D, Schultz G, Weiler E W
机构信息
Arbeitsgruppe Pflanzenphysiologie, Universität Osnabrück, FRG.
出版信息
Biochem Biophys Res Commun. 1988 Oct 31;156(2):954-9. doi: 10.1016/s0006-291x(88)80936-7.
Antisera raised against a highly conserved amino acid sequence (G alpha-common peptide) of animal Gs alpha, Gi alpha, Go alpha and Gt alpha recognize, in plasma membranes of several higher plants, sets of proteins of Mr = 37 and 31 kDa (Vicia faba), 36 and 31 kDa (Arabidopsis thaliana) and 38 and 34 kDa (Commelina communis). The A. thaliana proteins were solubilized and partially purified. They bind [35S]GTP gamma S with high affinity (apparent Kd approximately 10 nM) and, with lower affinity, GTP but not the other nucleotides tested (ATP, CTP, ITP, UTP).
用针对动物Gsα、Giα、Goα和Gtα的高度保守氨基酸序列（Gα通用肽）制备的抗血清，在几种高等植物的质膜中识别出分子量分别为37 kDa和31 kDa（蚕豆）、36 kDa和31 kDa（拟南芥）以及38 kDa和34 kDa（鸭跖草）的蛋白质组。拟南芥的蛋白质被溶解并部分纯化。它们以高亲和力（表观Kd约为10 nM）结合[35S]GTPγS，以较低亲和力结合GTP，但不结合其他测试的核苷酸（ATP、CTP、ITP、UTP）。
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