Atabey Mustafa, Tas Ayca, Sari Ismail, Bostanci Meric Emre, Topcu Omer, Silig Yavuz
Clinic of General Surgery, Medicana Sivas Hospital, Sivas, Turkey.
J BUON. 2019 May-Jun;24(3):1137-1142.
The Murine double minute 2 (MDM2) gene plays a crucial role in regulating and suppressing the function of apoptotic pathway. We investigated the relationship between MDM2 gene SNP309 (T309G) (rs2279744) polymorphism and colorectal cancer (CRC) in a Turkish population.
The polymorphism T309G (rs2279744) in the MDM2 gene was studied in patients with colorectal cancer (n=135) and healthy control subjects (n=145) using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The findings were evaluated using logistic regression and x2 tests.
When CRC cases and controls were evaluated based on different habits and family cancer histories, a statistically significant relationship was found between CRC and alcohol consumption (x2=4.07, p=0.044). Cancer cases and controls had statistically significant different family histories of cancer (x2=6.82, p=0.009). There was also significant difference in TG genotype distribution in the MDM2 T309G polymorphism between those with and without cancer (OR=1.98, 95% CI=1.98-3.91, x2=4.00, p=0.045).
The SNP309 polymorphism of the MDM2 gene is associated with increased CRC risk in the Turkish population.
小鼠双微体2（MDM2）基因在调节和抑制凋亡途径功能中起关键作用。我们在土耳其人群中研究了MDM2基因SNP309（T309G）（rs2279744）多态性与结直肠癌（CRC）之间的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对135例结直肠癌患者和145例健康对照者的MDM2基因T309G（rs2279744）多态性进行研究。使用逻辑回归和卡方检验对研究结果进行评估。
根据不同习惯和家族癌症病史对结直肠癌病例和对照进行评估时，发现结直肠癌与饮酒之间存在统计学显著关系（卡方=4.07，p=0.044）。癌症病例和对照在家族癌症病史方面存在统计学显著差异（卡方=6.82，p=0.009）。在MDM2 T309G多态性的TG基因型分布上，患癌者与未患癌者之间也存在显著差异（比值比=1.98，95%置信区间=1.98 - 3.91，卡方=4.00，p=0.045）。
MDM2基因的SNP309多态性与土耳其人群中结直肠癌风险增加相关。
