甲状旁腺激素及甲状旁腺激素片段对成骨细胞系细胞内游离钙离子浓度的影响。

Effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblast cell line.

作者信息

van Leeuwen J P, Bos M P, Löwik C W, Herrmann-Erlee M P

机构信息

Laboratory for Cell Biology and Histology, University of Leiden, The Netherlands.

出版信息

Bone Miner. 1988 Jun;4(2):177-88.

PMID:3142533

Abstract

In the present study the effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblastic cell line (UMR 106-01) is examined. In these cells the intact hormone, bPTH(1-84) and three fragments (bPTH(1-34), hPTH(1-34) and bPTH(3-34) induce an initial increase of the ionized calcium concentration [( Ca2+]i). The increase of the [Ca2+]i due to bPTH(1-84) is more pronounced and of longer duration compared to the increase due to PTH fragments. For two parathyroid hormone fragments with a modified amino acid sequence [( Nle-8,Nle-18,Tyr-34]bPTH(1-34), [Nle-8,Nle-18,Tyr-34]bPTH(3-34], no effect was found. Our observations suggest: (1) a second messenger role for intracellular ionized calcium in the action of bPTH(1-84) in osteoblasts; (2) a functional role for the COOH-terminal part in the action of bPTH(1-84); and (3) the existence of a specific part of the hormone being responsible for the increase of the intracellular ionized calcium concentration.

摘要

在本研究中，检测了甲状旁腺激素及甲状旁腺激素片段对成骨细胞系（UMR 106 - 01）细胞内游离钙浓度的影响。在这些细胞中，完整的激素bPTH(1 - 84)及三个片段（bPTH(1 - 34)、hPTH(1 - 34)和bPTH(3 - 34)）均可引起游离钙浓度[(Ca2 +]i)的初始升高。与甲状旁腺激素片段引起的升高相比，bPTH(1 - 84)引起的[Ca2 +]i升高更为显著且持续时间更长。对于两个氨基酸序列经修饰的甲状旁腺激素片段[（Nle - 8，Nle - 18，Tyr - 34）bPTH(1 - 34)、（Nle - 8，Nle - 18，Tyr - 34）bPTH(3 - 34)]，未发现有作用。我们的观察结果提示：(1) 细胞内游离钙在bPTH(1 - 84)对成骨细胞的作用中起第二信使作用；(2) COOH末端部分在bPTH(1 - 84)的作用中起功能作用；(3) 激素中存在一个特定部分负责细胞内游离钙浓度的升高。