米索前列醇治疗对阿霉素诱导的大鼠肾损伤的影响。
Effects of misoprostol treatment on doxorubicin induced renal injury in rats.
作者信息
Bilgic S, Armagan I
机构信息
Department of Medical Biochemistry, Vocational School of Health Services, University of Adıyaman, Adıyaman, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey.
出版信息
Biotech Histochem. 2020 Feb;95(2):113-120. doi: 10.1080/10520295.2019.1645356. Epub 2019 Aug 20.
We investigated the potential nephroprotective effects of misoprostol (MP) on doxorubicin (DOX) induced renal injury using histologic and biochemical assessment of rat kidneys. We used 21 male rats divided into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was given 0.5 ml 0.9% NaCl and 1 ml 0.9% NaCl orally for 6 days. DOX was administered as a single dose of 20 mg/kg on day 3. MP was administered orally for 6 days. We found that treatment with MP decreased serum creatinine and blood urea nitrogen levels significantly. DOX increased the malondialdehyde level and decreased catalase, superoxide dismutase activities and glutathione level. These alterations were prevented in renal tissues by MP. MP also decreased NADPH oxidase-4 and caspase-3 levels. In the DOX + MP group, oxidative stress was decreased, antioxidant activity was increased and histopathological changes were reduced compared to the DOX group. Renal injury caused by DOX was attenuated by MP treatment owing to the antioxidative and anti-apoptotic effects of MP.
我们通过对大鼠肾脏进行组织学和生化评估，研究了米索前列醇（MP）对阿霉素（DOX）诱导的肾损伤的潜在肾保护作用。我们将21只雄性大鼠分为三组：第一组为对照组；第二组为DOX组；第三组为DOX + MP组。对照组口服0.5 ml 0.9%氯化钠和1 ml 0.9%氯化钠，持续6天。在第3天给予DOX单剂量20 mg/kg。MP口服给药6天。我们发现，MP治疗可显著降低血清肌酐和血尿素氮水平。DOX增加了丙二醛水平，降低了过氧化氢酶、超氧化物歧化酶活性和谷胱甘肽水平。MP可防止肾组织出现这些改变。MP还降低了NADPH氧化酶-4和半胱天冬酶-3水平。与DOX组相比，DOX + MP组的氧化应激降低，抗氧化活性增加，组织病理学变化减少。由于MP的抗氧化和抗凋亡作用，MP治疗减轻了DOX引起的肾损伤。
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