Sandhiutami Ni Made Dwi, Desmiaty Yesi, Pitaloka Putu Diah Utari, Salsabila Salsabila
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia.
Departement of Phytochemistry, Faculty of Pharmacy, Pancasila University, Jakarta, Indonesia.
Res Pharm Sci. 2024 Oct 22;19(5):591-605. doi: 10.4103/RPS.RPS_99_23. eCollection 2024 Oct.
Doxorubicin chemotherapy is a widely used treatment for various cancers, including breast, ovarian, and uterine cancers, among others. However, long-term use can cause nephrotoxicity side effects. Some citrus flavonoids have demonstrated nephroprotective activity; therefore, this study aimed to test the nephroprotective effectiveness of peel extract in protecting and reducing kidney damage caused by doxorubicin.
peel was dried, ground, and extracted by ultrasonication (70% ethanol), then the extract was dried. Twenty-five female Sprague-Dawley rats were divided into 5 groups including the normal group (control), positive control (doxorubicin) group receiving doxorubicin at the repeated intraperitoneal (i.p.) dose of 4 mg/kg/day on days 2, 6, 10, and 14, and treatment groups receiving peel extract (CPE) with the doses of 100, 200, and 400 mg/kg/day orally for 14 days, and doxorubicin (4 mg/kg/day, i.p.) on days 2, 6, 10 and 14. On day 15, the rats were euthanized for the measurements of MDA, superoxide dismutase (SOD), catalase, kidney function (measuring blood urea nitrogen (BUN), creatinine, albumin serum levels), and renal histopathology.
FINDINGS/RESULTS: The CPE yield was 16.13%. CPE could significantly reduce the levels of MDA, and increase SOD and catalase activities compared with the doxorubicin-induced nephrotoxic model. CPE could increase renal function by reducing BUN and creatinine levels, increasing albumin, and improving the histopathology of the kidney.
CPE has a potential effect as nephroprotective against doxorubicin-induced toxicity in renal through antioxidant capacities and increased renal function.
阿霉素化疗是一种广泛用于治疗多种癌症的方法，包括乳腺癌、卵巢癌和子宫癌等。然而，长期使用会导致肾毒性副作用。一些柑橘类黄酮已显示出肾保护活性；因此，本研究旨在测试果皮提取物在保护和减少阿霉素引起的肾损伤方面的肾保护效果。
将果皮干燥、研磨，并用超声处理（70%乙醇）提取，然后将提取物干燥。25只雌性Sprague-Dawley大鼠分为5组，包括正常组（对照组）、阳性对照组（阿霉素组），阿霉素组在第2、6、10和14天以4mg/kg/天的重复腹腔内（i.p.）剂量接受阿霉素，治疗组分别以100、200和400mg/kg/天的剂量口服果皮提取物（CPE）14天，并在第2、6、10和14天接受阿霉素（4mg/kg/天，i.p.）。在第15天，对大鼠实施安乐死以测量丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶、肾功能（测量血尿素氮（BUN）、肌酐、血清白蛋白水平）以及肾脏组织病理学。
CPE产率为16.13%。与阿霉素诱导的肾毒性模型相比，CPE可显著降低MDA水平，并增加SOD和过氧化氢酶活性。CPE可通过降低BUN和肌酐水平、增加白蛋白以及改善肾脏组织病理学来提高肾功能。
CPE通过抗氧化能力和增强肾功能，对阿霉素诱导的肾脏毒性具有潜在的肾保护作用。
