角膜树突状细胞在稳态和炎症期间的拓扑和形态差异。
Topographical and Morphological Differences of Corneal Dendritic Cells during Steady State and Inflammation.
机构信息
Department of Optometry and Vision Sciences, The University of Melbourne , Melbourne, Australia.
Monash Genome Modification Platform, Faculty of Medicine, Nursing and Health Sciences, Monash University , Melbourne, Australia.
出版信息
Ocul Immunol Inflamm. 2020 Aug 17;28(6):898-907. doi: 10.1080/09273948.2019.1646775. Epub 2019 Aug 20.
PURPOSE
We report novel differences in mouse corneal DC morphology and density during local and systemic inflammation.
METHODS
Local inflammation was induced by topical application of saline or TLR9 agonist CpG-ODN on abraded C57BL6J mouse corneas. Systemic inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in CD11c-YFP mice. Corneal epithelial DCs from uninjured, injured and contralateral eyes were analysed by confocal microscopy.
RESULTS
Following local CpG delivery on the injured cornea, the DC density and size increased in both central and peripheral regions. Contralateral uninjured eyes displayed enlarged DC morphology in the central cornea compared to naïve cohorts. After systemic LPS, the size of DCs in the central cornea was lower at 2 hours, returning to baseline after 24 hours.
CONCLUSIONS
Corneal DCs respond differently in terms of shape and distribution during local and systemic inflammation. These features can serve as indicators in ocular and systemic diseases.
目的
我们报告了在局部和全身炎症期间，小鼠角膜树突状细胞（DC）形态和密度的新差异。
方法
通过在 C57BL6J 小鼠角膜上划痕后局部应用生理盐水或 TLR9 激动剂 CpG-ODN 来诱导局部炎症。通过腹腔内注射脂多糖（LPS）在 CD11c-YFP 小鼠中诱导全身炎症。通过共聚焦显微镜分析未受伤、受伤和对侧眼睛的角膜上皮 DC。
结果
在受伤角膜上局部给予 CpG 后，中央和周边区域的 DC 密度和大小均增加。与未处理组相比，对侧未受伤眼睛的中央角膜 DC 形态增大。在全身 LPS 后，中央角膜的 DC 大小在 2 小时时较低，24 小时后恢复到基线。
结论
角膜 DC 在局部和全身炎症期间在形状和分布方面表现出不同的反应。这些特征可以作为眼部和系统性疾病的指标。
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