The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life, Jiangsu Normal University, Shanghai Road 101, 221116 Xuzhou, China.
Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Bioresour Technol. 2019 Nov;292:121968. doi: 10.1016/j.biortech.2019.121968. Epub 2019 Aug 8.
Process fluctuation caused by temperature modification of anaerobic digestion is routinely monitored via operational parameters, such as pH and gas production, but these parameters are lagging on microbial community performance. In this study, C isotope fractionation in CH and CO of biogas together with microbial community dynamics were applied to evaluate process stability in response to temperature increment. Results showed that the weakening correlated links between Firmicutes affiliated families and Methanomicrobiaceae were found regarding temperature increase. In contrast, Methanosarcinaceae and Methanobacteriaceae strengthened their links with multiple bacterial groups. This suggests that the C isotope fractionation in CH can predict the collapse of certain microbial interconnections and process instability, the new reinforced microbial links directly reflect the microbial community redundancy for maintaining function of syntrophic populations.
工艺波动由厌氧消化的温度变化引起，通常通过运行参数（如 pH 值和产气量）进行监测，但这些参数滞后于微生物群落性能。在这项研究中，沼气中 CH 和 CO 的 C 同位素分馏以及微生物群落动态被应用于评估温度升高时的工艺稳定性。结果表明，随着温度的升高，与厚壁菌门相关的科与甲烷微菌科之间的关联减弱。相比之下，甲烷八叠球菌科和甲烷杆菌科加强了与多个细菌群的联系。这表明 CH 中的 C 同位素分馏可以预测某些微生物相互联系的崩溃和工艺不稳定，新的增强的微生物联系直接反映了微生物群落的冗余性，以维持共培养种群的功能。
