Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China. Department of Medical Laboratory, The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Shenzhen 518020, People's Republic of China.
Nanotechnology. 2019 Nov 29;30(48):485706. doi: 10.1088/1361-6528/ab3cde.
In this work, a novel label-free electrochemical biosensor is developed for the detection of galectin-1 (Gal-1) based on gold nanoparticle (AuNP) loaded octahedral CuO (CuO@Au) nanocomposites. The AuNPs on the surface of the CuO nanocrystals not only enhance the electrochemical performance, but also serve as the binding sites for the lactose ligand which can specifically bind with Gal-1. The CuO@Au nanocomposites provide the synergic effect of electrochemical signal amplification and lactose-galectin reaction as the recognition strategy. Under optimal conditions, the proposed biosensor exhibits a variation of electrochemical responses to different concentrations of Gal-1 ranging from 0.1 pg ml to 10 ng ml. This work presents an alternative electrochemical biosensor for the detection of tumor biomarkers based on a simple and economical lactose ligand incorporated CuO@Au biosensor platform.
在这项工作中，开发了一种基于负载金纳米粒子（AuNP）的八面体 CuO（CuO@Au）纳米复合材料的新型无标记电化学生物传感器，用于检测半乳糖凝集素-1（Gal-1）。CuO 纳米晶体表面上的 AuNPs 不仅增强了电化学性能，而且还作为乳糖配体的结合位点，该配体可以特异性地与 Gal-1 结合。CuO@Au 纳米复合材料提供了电化学信号放大和乳糖-半乳糖凝集素反应的协同作用，作为识别策略。在最佳条件下，所提出的生物传感器对不同浓度 Gal-1 的电化学响应表现出变化，范围从 0.1 pg ml 到 10 ng ml。这项工作提出了一种基于简单经济的乳糖配体掺入的 CuO@Au 生物传感器平台的肿瘤生物标志物检测的替代电化学生物传感器。
