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海洋细菌 KQ11 碱性 GH49 支链淀粉酶的性质及其在异麦芽低聚糖制备中的应用。

Characterization of an Alkaline GH49 Dextranase from Marine Bacterium KQ11 and Its Application in the Preparation of Isomalto-Oligosaccharide.

机构信息

Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.

Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University, Hefei 230039, China.

出版信息

Mar Drugs. 2019 Aug 19;17(8):479. doi: 10.3390/md17080479.

DOI:10.3390/md17080479
PMID:31430863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6723167/
Abstract

A GH49 dextranase gene DexKQ was cloned from marine bacteria KQ11. It was recombinantly expressed using an system. Recombinant DexKQ dextranase of 66 kDa exhibited the highest catalytic activity at pH 9.0 and 55 °C. kcat/Km of recombinant DexKQ at the optimum condition reached 3.03 s μM, which was six times that of commercial dextranase (0.5 s μM). DexKQ possessed a m value of 67.99 µM against dextran T70 substrate with 70 kDa molecular weight. Thin-layer chromatography (TLC) analysis showed that main hydrolysis end products were isomalto-oligosaccharide (IMO) including isomaltotetraose, isomaltopantose, and isomaltohexaose. When compared with glucose, IMO could significantly improve growth of and and inhibit growth of and . This is the first report of dextranase from marine bacteria concerning recombinant expression and application in isomalto-oligosaccharide preparation.

摘要

从海洋细菌 KQ11 中克隆了 GH49 支链淀粉酶基因 DexKQ。利用 系统进行了重组表达。重组 DexKQ 支链淀粉酶的分子量为 66 kDa，在 pH 9.0 和 55°C 下表现出最高的催化活性。在最佳条件下，重组 DexKQ 的 kcat/Km 值达到 3.03 s μM，是商业支链淀粉酶（0.5 s μM）的六倍。DexKQ 对分子量为 70 kDa 的支链淀粉 T70 底物的 m 值为 67.99 μM。薄层层析（TLC）分析表明，主要的水解末端产物是异麦芽低聚糖（IMO），包括异麦芽糖四糖、异麦芽糖五糖和异麦芽糖六糖。与葡萄糖相比，IMO 能显著促进 和 的生长，抑制 和 的生长。这是首次报道海洋细菌来源的支链淀粉酶在异麦芽低聚糖制备中的重组表达和应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/d24b138c2629/marinedrugs-17-00479-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/37a0c11e64ab/marinedrugs-17-00479-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/ddcfa5479689/marinedrugs-17-00479-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/0f0b485b541a/marinedrugs-17-00479-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/3153d8934421/marinedrugs-17-00479-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/48c06f679dba/marinedrugs-17-00479-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/d59813446090/marinedrugs-17-00479-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/e56c2f769039/marinedrugs-17-00479-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/0154d38721be/marinedrugs-17-00479-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/686ff975f73d/marinedrugs-17-00479-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/d24b138c2629/marinedrugs-17-00479-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/37a0c11e64ab/marinedrugs-17-00479-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/ddcfa5479689/marinedrugs-17-00479-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/0f0b485b541a/marinedrugs-17-00479-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/3153d8934421/marinedrugs-17-00479-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/48c06f679dba/marinedrugs-17-00479-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/d59813446090/marinedrugs-17-00479-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/e56c2f769039/marinedrugs-17-00479-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/0154d38721be/marinedrugs-17-00479-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/686ff975f73d/marinedrugs-17-00479-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cc21/6723167/d24b138c2629/marinedrugs-17-00479-g010.jpg

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