通过SWATH质谱法对脑脊液进行非靶向代谢组学相对定量分析

Untargeted Metabolomics Relative Quantification by SWATH Mass Spectrometry Applied to Cerebrospinal Fluid.

作者信息

Mendes Vera M, Coelho Margarida, Manadas Bruno

机构信息

CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.

Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, Coimbra, Portugal.

出版信息

Methods Mol Biol. 2019;2044:321-336. doi: 10.1007/978-1-4939-9706-0_20.

DOI:10.1007/978-1-4939-9706-0_20

PMID:31432422

Abstract

Cerebrospinal fluid (CSF) has been considered the key source for the search of biomarkers, in particular for neurological diseases, such as Alzheimer's and Parkinson's disease, since it reflects the state of the central nervous system (CNS). Finding biomarkers in the earliest stages of neurodegenerative diseases has become imperative, since, at the moment, there are no drugs that can reverse these pathological processes. Untargeted metabolomics analysis by liquid chromatography combined with SWATH-MS relative quantification is an emerging approach to search for potential biomarkers. In this chapter, we describe a method for untargeted metabolomics analysis of CSF samples that can also be used in parallel to a proteomics approach. The analysis is focused on the SWATH acquisition mode, where beyond precursor's relative quantification, the information of the MS/MS relative quantification is also used to help in the search of new potential biomarkers.

摘要

脑脊液（CSF）一直被认为是寻找生物标志物的关键来源，特别是对于神经疾病，如阿尔茨海默病和帕金森病，因为它反映了中枢神经系统（CNS）的状态。在神经退行性疾病的最早阶段寻找生物标志物变得势在必行，因为目前尚无药物能够逆转这些病理过程。液相色谱结合SWATH-MS相对定量的非靶向代谢组学分析是一种寻找潜在生物标志物的新兴方法。在本章中，我们描述了一种用于脑脊液样本非靶向代谢组学分析的方法，该方法也可与蛋白质组学方法并行使用。分析聚焦于SWATH采集模式，在此模式下，除了前体的相对定量外，MS/MS相对定量信息也用于帮助寻找新的潜在生物标志物。