人类 B1 细胞是主要的血型 A 特异性 B 细胞，与抗 A 抗体效价有中度相关性。
Human B1 Cells are the Main Blood Group A-Specific B Cells That Have a Moderate Correlation With Anti-A Antibody Titer.
机构信息
Department of Preventive Medicine, Yanbian University College of Medicine, Yanji, Jilin, People's Republic of China.
Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea.
出版信息
Ann Lab Med. 2020 Jan;40(1):48-56. doi: 10.3343/alm.2020.40.1.48.
BACKGROUND
Anti-carbohydrate antibody responses, including those of anti-blood group ABO antibodies, are yet to be thoroughly studied in humans. Because anti-ABO antibody-mediated rejection is a key hurdle in ABO-incompatible transplantation, it is important to understand the cellular mechanism of anti-ABO responses. We aimed to identify the main human B cell subsets that produce anti-ABO antibodies by analyzing the correlation between B cell subsets and anti-ABO antibody titers.
METHODS
Blood group A-binding B cells were analyzed in peritoneal fluid and peripheral blood samples from 43 patients undergoing peritoneal dialysis and 18 healthy volunteers with blood group B or O. The correlation between each blood group A-specific B cell subset and anti-A antibody titer was then analyzed using Pearson's correlation analysis.
RESULTS
Blood group A-binding B cells were enriched in CD27CD43CD1c B1, CD5 B1, CD11b B1, and CD27CD43CD1c marginal zone-B1 cells in peripheral blood. Blood group A-specific B1 cells (=0.029 and R=0.356 for IgM; =0.049 and R=0.325 for IgG) and marginal zone-B1 cells (=0.011 and R=0.410 for IgM) were positively correlated with anti-A antibody titer. Further analysis of peritoneal B cells confirmed B1 cell enrichment in the peritoneal cavity but showed no difference in blood group A-specific B1 cell enrichment between the peritoneal cavity and peripheral blood.
CONCLUSIONS
Human B1 cells are the key blood group A-specific B cells that have a moderate correlation with anti-A antibody titer and therefore constitute a potential therapeutic target for successful ABO-incompatible transplantation.
背景
抗碳水化合物抗体反应，包括抗血型 ABO 抗体，在人类中尚未得到充分研究。由于抗 ABO 抗体介导的排斥反应是 ABO 不相容移植的关键障碍，因此了解抗 ABO 反应的细胞机制很重要。我们旨在通过分析 B 细胞亚群与抗 ABO 抗体滴度之间的相关性，来确定产生抗 ABO 抗体的主要人类 B 细胞亚群。
方法
分析了 43 名接受腹膜透析的患者和 18 名血型 B 或 O 的健康志愿者的腹腔液和外周血样本中的血型 A 结合 B 细胞。然后使用 Pearson 相关分析分析了每个血型 A 特异性 B 细胞亚群与抗 A 抗体滴度之间的相关性。
结果
在外周血中，血型 A 结合 B 细胞富集于 CD27CD43CD1c B1、CD5 B1、CD11b B1 和 CD27CD43CD1c 边缘区-B1 细胞中。血型 A 特异性 B1 细胞（=0.029 和 R=0.356 用于 IgM；=0.049 和 R=0.325 用于 IgG）和边缘区-B1 细胞（=0.011 和 R=0.410 用于 IgM）与抗 A 抗体滴度呈正相关。对腹腔 B 细胞的进一步分析证实了腹腔内 B1 细胞的富集，但在腹腔和外周血之间，血型 A 特异性 B1 细胞的富集没有差异。
结论
人类 B1 细胞是产生与抗 A 抗体滴度中度相关的关键血型 A 特异性 B 细胞，因此构成成功 ABO 不相容移植的潜在治疗靶点。
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