Schremi W, Lohrmann H P
Cancer Res. 1979 Oct;39(10):4195-9.
The present studies were performed to delineate the effect of high-dose methotrexate with citrovorum factor rescue on the measurable compartments of human granulopoiesis, including peripheral blood cells and bone marrow stem cells committed to granulopoiesis-monocytopoiesis. Parameters of granulopoiesis were determined during the first course of high-dose methotrexate with citrovorum factor rescue in five patients, and during four consecutive courses in one patient. The remarkably mild toxicity of high-dose methotrexate with citrovorum factor rescue reported in clinical studies was substantiated on the level of the committed stem cell compartment. The data suggest a higher sensitivity of in vitro colony-forming units in agar culture as compared to the more mature granulopoietic cells and document the exquisite sensitivity and regenerating capacity of erythroid precursors after this type of chemotherapy.
进行本研究以阐明大剂量甲氨蝶呤联合亚叶酸解救对人类粒细胞生成可测量部分的影响，包括外周血细胞和致力于粒细胞-单核细胞生成的骨髓干细胞。在5例患者接受大剂量甲氨蝶呤联合亚叶酸解救的首个疗程期间，以及1例患者的连续4个疗程期间，测定粒细胞生成参数。临床研究报道的大剂量甲氨蝶呤联合亚叶酸解救的显著轻微毒性在定向干细胞部分得到证实。数据表明，与更成熟的粒细胞生成细胞相比，琼脂培养中的体外集落形成单位具有更高的敏感性，并证明了此类化疗后红系前体细胞具有极高的敏感性和再生能力。
