Guangdong Engineering and Technology Research Center for Ambient Mass Spectrometry, Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Institute of Analysis (China National Analytical Center Guangzhou), 100 Xianlie Middle Road, Guangzhou 510070, China.
State Key Laboratory of Biocontrol, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat-Sen University, 135 Xingangxi Road, Guangzhou 510275, China.
Analyst. 2019 Sep 21;144(18):5637-5645. doi: 10.1039/c9an01195a. Epub 2019 Aug 21.
The simultaneous analysis of perfluoroalkyl substances (PFASs) and lipids in biological tissues is of importance, especially for in situ and microscale analysis, because it provides significant information to understand the relevance of content, composition, and distribution of lipids to the bioaccumulation of PFASs as well as lipid metabolism affected by the biotoxicity of PFASs. In this study, we report the development of a novel ambient mass spectrometry method for the rapid, in situ, and microscale analysis of PFASs and lipids simultaneously in biological tissues for the investigation of their biological correlation. A microscale solid-phase microextraction (SPME) probe with a probe-end diameter of several-μm was employed for in situ and microscale sampling of biological tissues after PFAS exposure. The SPME probe showed a desirable capacity for the enrichment of PFASs and lipid species simultaneously. After sampling and extraction, the loaded SPME probe was directly applied for nanoESI-MS analysis under ambient and open-air conditions. A high-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometer operated in the field-induced mode was introduced to record mass spectra using fast polarity switching between positive and negative ion detection. Most of the lipid species were recorded in the positive ion mass spectrum, and PFASs were recorded in the negative ion mass spectrum. By using the developed method, the in situ analysis of PFASs and lipids in the muscle, brain, heart, kidney, liver, and intestine of zebrafish was realized. In addition, simultaneously imaging PFASs and lipids in individual Daphnia magna was successfully achieved for the investigation of their biological correlation.
同时分析生物组织中的全氟烷基物质 (PFASs) 和脂质非常重要，特别是对于原位和微尺度分析，因为它提供了有关脂质含量、组成和分布与 PFASs 生物积累以及受 PFASs 生物毒性影响的脂质代谢之间相关性的重要信息。在本研究中，我们报告了一种新的环境质谱方法的发展，用于快速、原位和微尺度分析生物组织中的 PFASs 和脂质，以研究它们的生物学相关性。采用几微米探针端直径的微尺度固相微萃取 (SPME) 探针，用于 PFAS 暴露后生物组织的原位和微尺度采样。SPME 探针显示出良好的同时富集 PFASs 和脂质种类的能力。采样和提取后，将加载的 SPME 探针直接用于在环境和开放空气条件下进行纳升电喷雾电离 - 质谱分析。引入了一种在现场诱导模式下运行的高分辨率傅里叶变换离子回旋共振 (FTICR) 质谱仪，用于通过正离子和负离子检测之间的快速极性切换记录质谱。大多数脂质种类记录在正离子质谱中，而 PFASs 则记录在负离子质谱中。使用所开发的方法，实现了斑马鱼肌肉、大脑、心脏、肾脏、肝脏和肠道中 PFASs 和脂质的原位分析。此外，还成功实现了对单个水蚤中 PFASs 和脂质的同时成像，以研究它们的生物学相关性。
