Oshima A, Tsuji A, Nagao Y, Sakuraba H, Suzuki Y
Department of Clinical Genetics, Tokyo Metropolitan Institute of Medical Science, Japan.
Biochem Biophys Res Commun. 1988 Nov 30;157(1):238-44. doi: 10.1016/s0006-291x(88)80038-x.
We cloned and sequenced the full-length cDNA for human placental beta-galactosidase. The 2379-nucleotide sequence contains 2031 nucleotides which encode a protein of 677 amino acids. The amino acid sequence includes a putative signal sequence of 23 amino acids and 7 potential asparagine-linked glycosylation sites. The cDNA in the expression vector pSVL was used to transfect COS cells. Expression of the cDNA in transfected COS cells produced immunoprecipitable proteins and led to an increase in beta-galactosidase activity.
我们克隆并测定了人胎盘β-半乳糖苷酶的全长cDNA序列。该2379个核苷酸的序列包含2031个核苷酸，编码一个由677个氨基酸组成的蛋白质。氨基酸序列包括一个23个氨基酸的推定信号序列和7个潜在的天冬酰胺连接的糖基化位点。用表达载体pSVL中的cDNA转染COS细胞。转染的COS细胞中cDNA的表达产生了可免疫沉淀的蛋白质，并导致β-半乳糖苷酶活性增加。
