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传染性喉气管炎病毒（ILTV）感染期间糖蛋白 G（gG）的产生情况。

Glycoprotein G (gG) production profile during infectious laryngotracheitis virus (ILTV) infection.

机构信息

Laboratorios de Investigación y Desarrollo-FARVET, Chincha Alta, Ica, Peru.

出版信息

PLoS One. 2019 Aug 21;14(8):e0219475. doi: 10.1371/journal.pone.0219475. eCollection 2019.

DOI:10.1371/journal.pone.0219475
PMID:31433806
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6703672/
Abstract

Glycoprotein G (gG) is a conserved protein, and it has been described as a chemokine-binding protein in most members of the alphaherpesviruses. In case of the infectious laryngotracheitis virus (ILTV), an alphaherpesvirus that infects chickens, this protein is a virulence factor that plays an immunomodulatory role in the chicken immune response. Nevertheless, the gG production profile during ILTV infection has not yet been studied. In this study, we developed monoclonal antibodies in order to determine the gG production profile during ILTV infection in chicken hepatocellular carcinoma (LMH) cell cultures as well as embryonated specific-pathogen-free (SPF) chicken eggs and SPF chickens using a sandwich enzyme-linked immunosorbent assay (ELISA). Despite the fact that inoculated LMH cell cultures showed an increase in both gG production and viral genome copy number up to 96 h after inoculation, we observed that gG production started earlier than the increase in viral genome copy number in ILTV infected embryonated SPF chicken eggs. Likewise, a gG production peak and an increase of viral genome copy number was observed prior to the appearance of clinical signs in infected SPF chickens. According to the production profiles, gG was also produced quite early in eggs and chickens inoculated with ILTV. These findings contribute to the knowledge of the gG role during the ILTV infection as a virulence factor.

摘要

糖蛋白 G (gG) 是一种保守的蛋白质，大多数α疱疹病毒都将其描述为趋化因子结合蛋白。在传染性喉气管炎病毒 (ILTV) 这种感染鸡的α疱疹病毒中，该蛋白是一种毒力因子，在鸡的免疫反应中发挥免疫调节作用。然而，ILTV 感染过程中 gG 的产生情况尚未得到研究。在本研究中，我们开发了单克隆抗体，以便使用夹心酶联免疫吸附测定 (ELISA) 在鸡肝癌 (LMH) 细胞培养物以及无特定病原体 (SPF) 鸡胚和 SPF 鸡中确定 ILTV 感染期间 gG 的产生情况。尽管接种的 LMH 细胞培养物在接种后 96 小时内显示出 gG 产生和病毒基因组拷贝数的增加，但我们观察到 gG 的产生早于 ILTV 感染的 SPF 鸡胚中病毒基因组拷贝数的增加。同样，在感染的 SPF 鸡出现临床症状之前，观察到 gG 产生峰值和病毒基因组拷贝数增加。根据产生情况，在接种 ILTV 的鸡蛋和鸡中 gG 也很早就产生了。这些发现有助于了解 gG 在作为毒力因子的 ILTV 感染过程中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/b3d343b9104b/pone.0219475.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/d886f1909366/pone.0219475.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/e96a8dc800ee/pone.0219475.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/68ee1625af1f/pone.0219475.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/df0b9e20a6ba/pone.0219475.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/9bbdd8e621c7/pone.0219475.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/9f984b2a695e/pone.0219475.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/fbac6b145513/pone.0219475.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/b3d343b9104b/pone.0219475.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/d886f1909366/pone.0219475.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/e96a8dc800ee/pone.0219475.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/68ee1625af1f/pone.0219475.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/df0b9e20a6ba/pone.0219475.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/9bbdd8e621c7/pone.0219475.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/9f984b2a695e/pone.0219475.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/fbac6b145513/pone.0219475.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1512/6703672/b3d343b9104b/pone.0219475.g008.jpg

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