Young M, Blanchard M H, Sessions F, Boyle M D
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610.
Biochemistry. 1988 Sep 6;27(18):6675-81. doi: 10.1021/bi00418a005.
Studies from several laboratories have shown that mouse submandibular glands and mouse saliva contain nerve growth factor (NGF) as part of a high molecular weight oligomeric macromolecule composed of three different subunits, termed alpha, beta, and gamma. The beta-subunit is the nerve growth-promoting protein. The gamma-subunit is a serine protease class enzyme of highly restricted substrate specificity. The alpha-subunit has no known function. This high molecular weight form of nerve growth factor is also a Zn(II)-containing metalloprotein. In the present study, measurements of multiple physicochemical parameters have been used to deduce the subunit structure of high molecular weight NGF. Results demonstrate that it contains two alpha-, one beta- and one gamma-subunit together with one tightly bound Zn(II) ion per molecule.
来自多个实验室的研究表明，小鼠颌下腺和小鼠唾液中含有神经生长因子（NGF），它是一种由三种不同亚基（称为α、β和γ）组成的高分子量寡聚大分子的一部分。β亚基是促进神经生长的蛋白质。γ亚基是一种底物特异性高度受限的丝氨酸蛋白酶类酶。α亚基的功能尚不清楚。这种高分子量形式的神经生长因子也是一种含锌（II）的金属蛋白。在本研究中，通过测量多个物理化学参数来推断高分子量NGF的亚基结构。结果表明，它每分子包含两个α亚基、一个β亚基和一个γ亚基，以及一个紧密结合的锌（II）离子。
