Hanke T, Tyers M, Harley C B
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
FEBS Lett. 1988 Dec 5;241(1-2):159-63. doi: 10.1016/0014-5793(88)81051-2.
Metallothionein (MT) gene transcription is regulated in a developmental and tissue-specific manner by metal ions and other agents. We examined MT expression in the human promyelocytic leukemia cell line HL-60 during differentiation along macrophage and neutrophil lineages. All HL-60 phenotypes showed similar basal levels of MT RNA with significant induction following exposure to Cd2+ but not activators of PKC. MT RNA did not correlate with growth state or with known levels of PKC activity, thus our data do not support a role for MT in HL-60 differentiation or for PKC in MT expression.
金属硫蛋白（MT）基因转录受金属离子和其他因子以发育和组织特异性方式调控。我们检测了人早幼粒细胞白血病细胞系HL-60在沿巨噬细胞和中性粒细胞谱系分化过程中的MT表达。所有HL-60表型均显示出相似的MT RNA基础水平，暴露于Cd2+后有显著诱导，但PKC激活剂处理后无此现象。MT RNA与生长状态或已知的PKC活性水平无关，因此我们的数据不支持MT在HL-60分化中起作用，也不支持PKC在MT表达中起作用。
