März W, Trommlitz M, Gross W
Gustav Embden-Zentrum der biologischen Chemie, Johann Wolfgang Goethe-Universität, Frankfurt/Main, FRG.
J Clin Chem Clin Biochem. 1988 Sep;26(9):573-8. doi: 10.1515/cclm.1988.26.9.573.
A differential immunoturbidimetric procedure for the quantitation of apolipoprotein A-I associated with lipoproteins LpA (containing both apolipoprotein A-I and apolipoprotein A-II) and with lipoproteins LpA-I (containing apolipoprotein A-I but no apolipoprotein A-II) is presented. Lipoproteins containing apolipoprotein A-II are precipitated with an anti-apolipoprotein A-II antibody. The resulting immunoprecipitate is sedimented and LpA-IA-I is measured in the supernate. Whereas LpA-IA-I concentrations differed significantly between normolipidaemic men and women (0.75 and 1.00 g/l, respectively), there was virtually no sex related difference in LpAA-I (0.83 and 0.88 g/l, respectively). LpA-IA-I was predominantly correlated with HDL2-cholesterol (rs = 0.630), whereas LpAA-I was statistically associated with HDL3 (rs = 0.417).
本文介绍了一种用于定量与脂蛋白LpA（同时含有载脂蛋白A-I和载脂蛋白A-II）以及脂蛋白LpA-I（仅含载脂蛋白A-I而不含载脂蛋白A-II）相关的载脂蛋白A-I的差示免疫比浊法。含载脂蛋白A-II的脂蛋白用抗载脂蛋白A-II抗体沉淀。将所得免疫沉淀物沉淀，然后测定上清液中的LpA-IA-I。正常血脂男性和女性的LpA-IA-I浓度存在显著差异（分别为0.75和1.00 g/l），而LpAA-I实际上不存在性别相关差异（分别为0.83和0.88 g/l）。LpA-IA-I主要与HDL2-胆固醇相关（rs = 0.630），而LpAA-I与HDL3存在统计学关联（rs = 0.417）。
